Publication

Microsecond melting and revitrification of cryo samples with a correlative light-electron microscopy approach

Related publications (37)

2dx_automator: Implementation of a semiautomatic high-throughput high-resolution cryo-electron crystallography pipeline

The introduction of direct electron detectors (DED) to cryo-electron microscopy has tremendously increased the signal-to-noise ratio (SNR) and quality of the recorded images. We discuss the optimal use of DEDs for cryo-electron crystallography, introduce a ...

Elsevier BV2014

Single-cell lysis for visual analysis by electron microscopy

Henning Paul-Julius Stahlberg

The stochastic nature of biological systems makes the study of individual cells a necessity in systems biology. Yet, handling and disruption of single cells and the analysis of the relatively low concentrations of their protein components still challenges ...

Elsevier BV2013

Universal sample holder

Graham Knott

This device is for holding samples during their preparation prior to imaging in the electron microscope. The design means it can be transferred between the light and electron microscopes as well as trimming devices used to prepare the final sample. It can ...

2013

Assessing the benefits of focal pair cryo-electron tomography

Henning Paul-Julius Stahlberg

Cryo electron tomography provides nanometer-scale information on biological matter preserved in a close-to native state. The resolution of tomograms and structures resolved by sub-tomogram averaging is typically limited by the contrast transfer function of ...

Elsevier BV2012

Efficient Scanning for EM Based Target Localization

Pascal Fua, Graham Knott, Raphael Sznitman, Aurélien Lucchi, Natasa Pjescic-Emedi

For biologists studying the morphology of cells, Electron Microscopy (EM) is the method of choice with its {\it nm} resolution, across increasingly larger volumes. However, the time necessary to acquire such image series is long and often limits the amount ...

2012

W-2 wt.%Y2O3 composite: Microstructure and mechanical properties

Philippe Spätig, Nadine Baluc, Robin Schäublin

A W-2Y(2)O(3) composite is produced by powder metallurgy, including the pressing of the mixed elemental powders, their sintering and hot forging. The microstructure of the obtained composite is investigated using light microscopy, scanning electron microsc ...

2012

Reverse precipitation synthesis and characterization of CeO2 nanopowder

Mehdi Mazaheri

In the present work, CeO2 nanopowder was synthesized via a reverse precipitation method using CeCl3 center dot 7H(2)O, NH4OH and sodium dodecyl sulfate as raw materials. The effect of thermal treatment on the crystal growth, surface area and chemical bonds ...

2010

An optical and microPET assessment of thermally-sensitive liposome biodistribution in the Met-1 tumor model: Importance of formulation

Henning Paul-Julius Stahlberg, Hua Zhang, Jin Won Seo

The design of delivery vehicles that are stable in circulation but can be activated by exogenous energy sources is challenging. Our goals are to validate new imaging methods for the assessment of particle stability, to engineer stable and activatable parti ...

Elsevier BV2010

Magnetic circular dichroism in electron microscopy

Cécile Hébert

Although the analogies between the electron energy loss spectra and X-ray absorption spectra were recognized long ago, the possibility to observe the magnetic circular dichroism in the electron microscope was demonstrated only recently. This technique comb ...

2008

Photoluminescence characterization of localized 2D-layers of few-nanocrystals electronic devices

Marc Antonius Friedrich van den Boogaart

Photoluminescence (PL) spectroscopy has been demonstrated as a suitable technique to characterize Si nanocrystal-based non-volatile memory devices (Carrada et al. APL 2005). These are formed by a single plane of Si nanocrystals (NCs) buried in the gate oxi ...

2006

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