Publications related to In Situ and Time-Resolved Transmission Electron Microscopy of Nanoscale Processes

Bending modes of DNA directly addressed by cryo-electron microscopy of DNA minicircles

Davide Demurtas, John Maddocks, Andrzej Stasiak, Jacques Dubochet, Arnaud Amzallag

We use cryo-electron microscopy (cryo-EM) to study the 3D shapes of 94-bp-long DNA minicircles and address the question of whether cyclization of such short DNA molecules necessitates the formation of sharp, localized kinks in DNA or whether the necessary ...

Oxford University Press2009

A Novel Method for the Encapsulation of Biomolecules into Polymersomes via Direct Hydration

Davide Demurtas, Jeffrey Alan Hubbell, Conlin O'Neil, Andrija Finka

One of the major engineering challenges for the implementation of block copolymer vesicles, or polymersomes, as therapeutic drug carriers is obtaining high encapsulation efficiencies for biomolecules. Here we present it novel method for encapsulation of pr ...

2009

Particle Size Distribution Measurements of Manganese-Doped ZnS Nanoparticles

Heinrich Hofmann, Alke Fink

We performed particle size and particle size distribution measurements for L-cysteine-stabilized ZnS/Mn nanoparticles in the size region below 10 nm. For this we applied transmission electron microscopy (TEM), analytical ultracentrifugation (AUC), dynamic ...

2009

On Random Tomography with Unobservable Projection Angles

Victor Panaretos

We formulate and investigate a statistical inverse problem of a random tomographic nature, where a probability density function on

\mathbb{R}^3

is to be recovered from observation of finitely many of its two-dimensional projections in random and unobserv ...

Institute of Mathematical Statistics2009

Microstructure of precipitates and magnetic domain structure in an annealed Co38Ni33Al29 shape memory alloy

Barbora Bártová

The microstructure of a Co38Ni33Al29 ferromagnetic shape memory alloy was determined by conventional transmission electron microscopy (TEM), electron diffraction studies together with advanced microscopy techniques and in situ Lorentz microscopy. Rod-like ...

2008

Visualization of proteins in intact cells with a clonable tag for electron microscopy

Paul Guichard

Identification of proteins in 3D maps of cells is a main challenge in structural cell biology. For light microscopy (LM) clonable reagents such as green fluorescent protein represented a real revolution and equivalent reagents for transmission electron mic ...

2008

Silicon nanoparticles synthesized in SiO2 pockets by stencil-masked low energy ion implantation and thermal annealing

Jürgen Brugger, Marc Antonius Friedrich van den Boogaart

We propose an original approach called a "stencil-masked ion implantation process" for performing a spatially localized synthesis of a limited number of Si nanocrystals within a thin SiO2 layer. In this process, the SiO2 layer is irradiated with 1 keV sili ...

Elsevier2008

Microstructure of 5 keV gold-implanted polydimethylsiloxane

Samuel Rosset, Muhamed Niklaus

The first high-resolution transmission electron microscopy (TEM) cross-section images of flexible electrodes fabricated by gold ion implantation at 5 keV into polydimethylsiloxane (PDMS) are presented. A TEM sample preparation method based on cryoultramicr ...

Elsevier2008

Enhanced x-ray irradiation-induced cancer cell damage by gold nanoparticles treated by a new synthesis method of polyethylene glycol modification

Giorgio Margaritondo

We explored a very interesting gold nanoparticle system-pegylated gold in colloidal solution-and analyzed its uptake by mice colorectal adenocarcinoma CT26 tumor cells and the impact on the cell's response to x-ray irradiation. We found that exposure to po ...

Institute of Physics2008

Orientation selection in dendritic evolution

Michel Rappaz

Computational and experimental demonstration of dendritic crystal growth direction and the morphologic diversity of the resulting dendritic structures are discussed. The primary dendrite growth directions can vary continuously between different crystallogr ...

2008

In Situ and Time-Resolved Transmission Electron Microscopy of Nanoscale Processes

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Bending modes of DNA directly addressed by cryo-electron microscopy of DNA minicircles

A Novel Method for the Encapsulation of Biomolecules into Polymersomes via Direct Hydration

Particle Size Distribution Measurements of Manganese-Doped ZnS Nanoparticles

On Random Tomography with Unobservable Projection Angles

Microstructure of precipitates and magnetic domain structure in an annealed Co38Ni33Al29 shape memory alloy

Visualization of proteins in intact cells with a clonable tag for electron microscopy

Silicon nanoparticles synthesized in SiO2 pockets by stencil-masked low energy ion implantation and thermal annealing

Microstructure of 5 keV gold-implanted polydimethylsiloxane

Enhanced x-ray irradiation-induced cancer cell damage by gold nanoparticles treated by a new synthesis method of polyethylene glycol modification

Orientation selection in dendritic evolution

Particle Size Distribution Measurements of Manganese-Doped ZnS Nanoparticles

Bending modes of DNA directly addressed by cryo-electron microscopy of DNA minicircles

A Novel Method for the Encapsulation of Biomolecules into Polymersomes via Direct Hydration

On Random Tomography with Unobservable Projection Angles

Microstructure of precipitates and magnetic domain structure in an annealed Co38Ni33Al29 shape memory alloy

Silicon nanoparticles synthesized in SiO2 pockets by stencil-masked low energy ion implantation and thermal annealing

Visualization of proteins in intact cells with a clonable tag for electron microscopy

Enhanced x-ray irradiation-induced cancer cell damage by gold nanoparticles treated by a new synthesis method of polyethylene glycol modification

Orientation selection in dendritic evolution

Microstructure of 5 keV gold-implanted polydimethylsiloxane