Publication
A field-capable rapid plant DNA extraction protocol using microneedle patches for botanical surveying and monitoring
Related publications (36)
Bypassing bacterial infection in phage display by sequencing DNA released from phage particles
Christian Heinis, Xudong Kong, Camille Villequey
Phage display relies on a bacterial infection step in which the phage particles are replicated to perform multiple affinity selection rounds and to enable the identification of isolated clones by DNA sequencing. While this process is efficient for wild-typ ...
2017On the statistical physics of chains and rods, with application to multi-scale sequence-dependent DNA modelling
The complex mechanisms involved in cellular processes have been increasingly understood this past century and the central role of the DNA molecule has been recognized. The base pair sequence along a DNA fragment is observed not only to encode the genomic i ...
EPFL2016Toward an Effective Control of DNA's Submolecular Conformation on a Surface
Giovanni Dietler, Dusan Vobornik, Aleksandre Japaridze, Andrea Cerreta, Ewelina Wioletta Lipiec
DNA adopts different conformations based on its environment. We reveal conditions that either preserve the DNA's physiological B-conformation, even upon its placement in UHV, or lead to a partial B-form to A-form reorganization upon DNA's deposition on a s ...
American Chemical Society2016Impedance sensing of DNA immobilization and hybridization by microfabricated alumina nanopore membranes
Jürgen Brugger, Philippe Renaud, Songmei Wu, Robert Meissner, Mojtaba Taghipoor
In this work we demonstrate microfabricated thin alumina nanopore membranes as a platform for impedance sensing of DNA immobilization and hybridization. We develop a wafer-scale fabrication of free-standing alumina nanopore membranes with well controlled t ...
Elsevier Science Sa2015Detecting the translocation of DNA through a nanopore using graphene nanoribbons
Aleksandra Radenovic, Andras Kis, Ke Liu, Daria Krasnozhon, Camille Alice Raillon, Floriano Traversi, Mohamed Malik Benameur, Sergey Khlybov
Solid-state nanopores can act as single-molecule sensors and could potentially be used to rapidly sequence DNA molecules. However, nanopores are typically fabricated in insulating membranes that are as thick as 15 bases, which makes it difficult for the de ...
Nature Publishing Group2013PyroTRF-ID: a novel bioinformatics methodology for the affiliation of terminal-restriction fragments using 16S rRNA gene pyrosequencing data
Christof Holliger, Julien Maillard, Pierre Rossi, Jacques Rougemont, David Gregory Weissbrodt, Noam Shani, Lucas Sinclair
The PyroTRF-ID bioinformatics methodology (http://bbcf.epfl.ch/PyroTRF-ID/) was developed to combine pyrosequencing and T-RFLP for describing microbial communities and identifying T-RFs by comparison of experimental and digital T-RFLP profiles obtained fro ...
2013Automated DNA purification from soil samples using Maxwell® 16
The isolation of high quality DNA from environmental samples such as soil is essential for use in downstream applications. Two common issues often encountered with the purification of DNA from soil are the ability to fully lyse microbial organisms in the s ...
2012Live-Cell dSTORM of Cellular DNA Based on Direct DNA Labeling
Suliana Manley, Aleksandr Benke
We have implemented the super-resolution method of direct stochastic optical reconstruction microscopy (dSTORM) to image nuclear and mitochondrial DNA in living cells. We also demonstrate time-lapse imaging, all using a dye that associates directly with ce ...
Wiley-Blackwell2011Interdigitated electrode DNA biosensor for detection of food-borne pathogens by impedance spectroscopy
The commercial application of biosensors has had a significant impact in a number of areas, particularly in the field of medical diagnostics. But in spite of the great number of publications, only a few systems are commercially available in food analysis. ...
EPFL2009New insights for using self-assembly materials to improve the detection stability in label-free DNA-chip and immuno-sensors
Sandro Carrara, Luca Benini, Andrea Cavallini, Anna Ferretti, Bruno Samori
This paper examines reliable advancements in low-cost DNA- and immuno-chips. Capacitance detection was successfully chosen to develop label-free bio-chips. Probe immobilization was rigorously investigated in order to obtain reliable capacitance measurement ...
Elsevier2009