Publication
Bioengineering human mini-intestines with in vivo-like complexity and function
Related publications (58)
Accessible and highly observable gastrointestinal organoid model systems for studying host-pathogen interactions
Traditional cell cultures have long been fundamental to biological research, offering an alternative to animal models burdened by ethical constraints and procedural intricacies, often lacking relevance to human physiology and disease. Moreover, their inabi ...
EPFL2024Bioengineering functional organoid models across scales
The last two decades have seen the development of organoid models for many different tissues and organs. Organoids are three-dimensional organ-mimetics derived from stem or progenitor cells comprising various specialized cell types, resembling the architec ...
EPFL2024Engineering hydrogel microenvironments for epithelial organoid culture
Organoids, miniature tissues generated from self-organizing stem cells within three-dimensional (3D) extracellular matrices (ECM), have opened up exciting possibilities for in vitro studies of complex physiological processes. A key factor in the success of ...
EPFL2024Bioengineered Tubular Biliary Organoids
Matthias Lütolf, Bilge Sen Elçi, Saba Rezakhani, Mikhail Nikolaev
Liver organoids have emerged as promising in vitro models for toxicology, drug discovery, and disease modeling. However, conventional 3D epithelial organoid culture systems suffer from significant drawbacks, including limited culture duration, a nonphysiol ...
Hoboken2024Annotation of spatially resolved single-cell data with STELLAR
Accurate cell-type annotation from spatially resolved single cells is crucial to understand functional spatial biology that is the basis of tissue organization. However, current computational methods for annotating spatially resolved single-cell data are t ...
2022Organoids as tools for fundamental discovery and translation-A Keystone Symposia report
Complex three-dimensional in vitro organ-like models, or organoids, offer a unique biological tool with distinct advantages over two-dimensional cell culture systems, which can be too simplistic, and animal models, which can be too complex and may fail to ...
WILEY2022Engineering Modular 3D Liver Culture Microenvironments In Vitro to Parse the Interplay between Biophysical and Biochemical Microenvironment Cues on Hepatic Phenotypes
In vitro models of human liver functions are used across a diverse range of applications in preclinical drug development and disease modeling, with particular increasing interest in models that capture facets of liver inflammatory status. This study invest ...
WILEY-V C H VERLAG GMBH2022Multiscale light-sheet organoid imaging framework
Petr Strnad, Andrea Boni, Raphaël Ortiz
Live imaging of organoid growth remains a challenge: it requires long-term imaging of several samples simultaneously and dedicated analysis pipelines. Here the authors report an experimental and image processing framework to turn long-term light-sheet imag ...
NATURE PORTFOLIO2022Developing new tools to study and program cell fate at the single-cell level
Single-cell transcriptomics (scRNA-seq) started a technological revolution in biology by enabling through the plethora of methods to assess a molecular state of the cell on systems level without the strict necessity of the prior knowledge of the cell state ...
EPFL2021Intestinal organoid cocultures with microbes
This protocol comprises various methods to coculture organoids (particularly human small intestinal and colon organoids) with microbes, including microinjection into the lumen and periphery of 3D organoids and exposure of organoids to microbes in a 2D laye ...
NATURE PORTFOLIO2021