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Microsecond Time-Resolved Cryo-Electron Microscopy
Related publications (267)
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Wiley-V C H Verlag Gmbh2024Structure-function analysis of the cyclic β-1,2-glucan synthase from Agrobacterium tumefaciens
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Wiley-V C H Verlag Gmbh2024Microsecond time-resolved cryo-electron microscopy
Microsecond time-resolved cryo-electron microscopy has emerged as a novel approach for directly observing protein dynamics. By providing microsecond temporal and near-atomic spatial resolution, it has the potential to elucidate a wide range of dynamics tha ...
2024Targeting TDP-43 Proteinopathies in Neurodegeneration by its Native-State Stabilization.
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Seventh pandemic Vibrio cholerae strains contain two pathogenicity islands that encode the DNA defense modules DdmABC and DdmDE. Here we use cryogenic electron microscopy to reveal the mechanistic basis for plasmid defense by DdmDE. A structure of the DdmD ...
2024Towards near-atomic resolution imaging of microsecond nanoscale dynamics
Time-resolved electron microscopy has made significant progress in recent years, with some groups now working on instruments that offer attosecond temporal resolution. While much of the research in the field revolves around the improvement of temporal reso ...
EPFL2023In Situ and Time-Resolved Transmission Electron Microscopy of Nanoscale Processes
Observing the fast dynamics of nanoscale systems is crucial in order to understand and ultimately control their behavior. Characterizing these dynamic processes requires techniques with atomic spatial resolution and a temporal resolution that matches the t ...
EPFL2023Fast viral dynamics revealed by microsecond time-resolved cryo-EM
Marcel Drabbels, Ulrich Lorenz, Sarah Victoria Barrass, Oliver Florian Harder
Observing proteins as they perform their tasks has largely remained elusive, which has left our understanding of protein function fundamentally incom- plete. To enable such observations, we have recently proposed a technique that improves the time resoluti ...
2023Near-atomic resolution reconstructions from in situ revitrified cryo samples
Marcel Drabbels, Ulrich Lorenz, Gabriele Bongiovanni, Jonathan Mark Voss, Oliver Florian Harder
A microsecond time-resolved version of cryo-electron microscopy (cryo-EM) has recently been introduced to enable observation of the fast conformational motions of proteins. The technique involves locally melting a cryo sample with a laser beam to allow the ...
2023