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Monitoring an Emergent Pathogen at Low Incidence in Wastewater Using qPCR: Mpox in Switzerland

Related concepts (42)
Pontederia crassipes
Pontederia crassipes (formerly Eichhornia crassipes), commonly known as common water hyacinth is an aquatic plant native to South America, naturalized throughout the world, and often invasive outside its native range. It is the sole species of the subgenus Oshunae within the genus Pontederia. Anecdotally, it is known as the "terror of Bengal" due to its invasive growth tendencies. Water hyacinth is a free-floating perennial aquatic plant (or hydrophyte) native to tropical and subtropical South America.
TaqMan
TaqMan probes are hydrolysis probes that are designed to increase the specificity of quantitative PCR. The method was first reported in 1991 by researcher Kary Mullis at Cetus Corporation, and the technology was subsequently developed by Hoffmann-La Roche for diagnostic assays and by Applied Biosystems (now part of Thermo Fisher Scientific) for research applications. The TaqMan probe principle relies on the 5 ́–3 ́ exonuclease activity of Taq polymerase to cleave a dual-labeled probe during hybridization to the complementary target sequence and fluorophore-based detection.

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