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Decoding Chromatin Ubiquitylation: A Chemical Biology Perspective
Related publications (65)
Dynamic protein-chromatin interactions in heterochromatin and during the DNA damage response on the single molecule level
Dynamic regulation of chromatin, a structure consisting of DNA and histone proteins, is mediated through histone post-translational modifications (PTMs) and effector proteins. HP1a and PRC2 are recruited to repressive PTMs (H3K9me3, H3K27me3) and enable he ...
EPFL2018Deciphering the chromatin binding mechanisms of human HP1 variants at the single-molecule level
In eukaryotic cells, DNA is tightly packed in the form chromatin. The basic structure of chromatin is a nucleosome composed of 147 bp DNA wrapped around eight histone proteins; two copies of H2A, H2B, H3 and H4. These histone proteins are decorated with pa ...
EPFL2018Single-molecule FRET reveals multiscale chromatin dynamics modulated by HP1α
Beat Fierz, Louise Catherine Bryan, Sinan Kilic, Iuliia Boichenko
The dynamic architecture of chromatin fibers, a key determinant of genome regulation, is poorly understood. Here, we employ multimodal single-molecule Förster resonance energy transfer studies to reveal structural states and their interconversion kinetics ...
2018A bi-terminal protein ligation strategy to probe chromatin structure during DNA damage
Beat Fierz, Sinan Kilic, Iuliia Boichenko, Carolin Christine Lechner
The cellular response to DNA damage results in a signaling cascade that primes chromatin for repair. Combinatorial post-translational modifications (PTMs) play a key role in this process by altering the physical properties of chromatin and recruiting downs ...
2018What shapes eukaryotic transcriptional bursting?
Felix Naef, Damien Lionel Nicolas, Nicholas Edward Phillips
Isogenic cells in a common environment present a large degree of heterogeneity in gene expression. Part of this variability is attributed to transcriptional bursting: the stochastic activation and inactivation of promoters that leads to the discontinuous p ...
Royal Society of Chemistry2017Rhythmic modulation of transcriptional burst frequency in circadian gene promoters
Isogenic cells sharing a common environment present a large degree of heterogeneity in gene expression, and stochasticity inherent to transcription substantially participates in this cell-to-cell variability. Notably, a majority of mammalian genes are tran ...
EPFL2017Rhythmic and clock-dependent chromatin loops regulate circadian gene expression
In mammals the circadian clock drives daily behavioural and physiological changes that resonate with environmental cues, which can be observed, for example, in the intricate timing of rest during the night and activity during the day in humans. The circadi ...
EPFL2017Single-molecule kinetic analysis of HP1-chromatin binding reveals a dynamic network of histone modification and DNA interactions
Vassily Hatzimanikatis, Georg Fantner, Beat Fierz, Sandrine Madeleine Suzanne Georgeon, Daniel Robert Weilandt, Oliver Hantschel, Louise Catherine Bryan, Pascal Damian Odermatt, Sinan Kilic, Andreas Linus Bachmann, Carolin Christine Lechner
Chromatin recruitment of effector proteins involved in gene regulation depends on multivalent interaction with histone post-translational modifications (PTMs) and structural features of the chromatin fiber. Due to the complex interactions involved, it is c ...
Oxford University Press (OUP)2017Heterochromatin protein 1 recruitment and chromatin conformational dynamics at the single-molecule level
Chromatin is the template on which DNA-associated transactions take place in eukaryotic organisms. Nucleosomes consisting of the four histones H2A, H2B, H3 and H4 each organize ~150bp of DNA and constitute a first layer of chromatin. The three-dimensional ...
EPFL2017The chromatin remodeling factor ISW-1 integrates organismal responses against nuclear and mitochondrial stress
Johan Auwerx, Maroun Bou Sleiman
Age-associated changes in chromatin structure have a major impact on organismal longevity. Despite being a central part of the ageing process, the organismal responses to the changes in chromatin organization remain unclear. Here we show that moderate dist ...
Nature Publishing Group2017