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Development of tools and methods for protein identification: From single molecules to in vivo applications.

Related publications (57)

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Proteome Digestion Specificity Analysis for Rational Design of Extended Bottom-up and Middle-down Proteomics Experiments

Yury Tsybin, Kristina Srzentic, Unige Anna Laskay

Mass spectrometry (MS)-based bottom-up proteomics (BUP) is currently the method of choice for large-scale identification and characterization of proteins present in complex samples, such as cell lysates, body fluids, or tissues. Technically, BUP relies on ...

Amer Chemical Soc2013

Structural Studies and Protein Engineering of Human O6-Alkylguanine-DNA Alkyltransferase

Birgit Mollwitz

The specific labeling of proteins with synthetic probes is a powerful approach to study protein function and protein tags have been widely used for this purpose. A well-established example for a self-labeling protein tag is SNAP-tag. It specifically reacts ...

EPFL2012

Quantitative fluorescence loss in photobleaching for analysis of protein transport and aggregation

Daniel Sage

Background: Fluorescence loss in photobleaching (FLIP) is a widely used imaging technique, which provides information about protein dynamics in various cellular regions. In FLIP, a small cellular region is repeatedly illuminated by an intense laser pulse, ...

Biomed Central Ltd2012

Characterization of semisynthetic and naturally Nα-acetylated α-synuclein in vitro and in intact cells: implications for aggregation and cellular properties of α-synuclein

Hilal Lashuel, Bruno Claude Daniel Fauvet, Mohamed Bilal Fares

N-terminal acetylation is a very common post-translational modification, although its role in regulating protein physical properties and function remains poorly understood. α-Synuclein (α-syn), a protein that has been linked to the pathogenesis of Parkinso ...

Amer Soc Biochemistry Molecular Biology Inc2012

Development of Electrokinetically Based Two-Dimensional Separation Methodologies for Proteomic Studies

Mohammad Reza Pourhaghighi

Proteomic studies require analytical methods capable of separating, identifying and quantifying thousands of proteins. Consequently, developing separation methodologies with high resolving power necessary to separate complex protein samples prior to protei ...

EPFL2012

The ability to specifically attach chemical probes to individual proteins represents a powerful approach to the study and manipulation of protein function in living cells. It provides a simple, robust and versatile approach to the imaging of fusion protein ...

2011

We present a fluorescence activation-coupled protein labeling (FAPL) method, which employs small-molecular probes that exhibit almost no basal fluorescence but acquire strong fluorescence upon covalent binding to tag-proteins. This method enables real-time ...

2011

Semisynthetic Fluorescent Sensor Proteins Based on Self-Labeling Protein Tags

Matthias Albert Brun

Genetically encoded fluorescent sensor proteins offer the possibility to probe the concentration of key metabolites in living cells. The approaches currently used to generate such fluorescent sensor proteins lack generality, as they require a protein that ...

EPFL2011

Photoactivatable Probes for Protein Labeling

Sambashiva Banala

Over the last decade, several protein tags have been developed for the specific and covalent labeling of fusion proteins with small organic probes. Among them, SNAP-tag has been used in many applications such as live cell imaging, protein microarrays and t ...

EPFL2010

We review new and established methods for the chemical modification of proteins in living cells and highlight recent applications. The review focuses on tag-mediated protein labeling methods, such as the tetracysteine tag and SNAP-tag, and new developments ...

Elsevier2010