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Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Accounting for these features is often critical in quantitatively interpreting microscopy images, especially those gathering information at scales on par with or smaller than light's emission wavelength. Here the optics responsible for generating fluorescent images, fluorophore properties, and microscopy modalities leveraging properties of both light and fluorophores, in addition to the necessarily probabilistic modeling tools imposed by the stochastic nature of light and measurement, are reviewed.
Edoardo Charbon, Claudio Bruschini, Ivan Michel Antolovic
Aleksandra Radenovic, Georg Fantner, Vytautas Navikas, Adrien Charles-François Raymond Descloux, Samuel Mendes Leitão, Kristin Stefanie Grussmayer
Michaël Unser, Thanh-An Michel Pham, Emmanuel Emilien Louis Soubies, Ferréol Arnaud Marie Soulez