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Spline projection-based volume-to-image registration

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3D Poses Recovery in Single-Particle Cryo-EM from Learned Pairwise Projection Distances

Jelena Banjac

Single-particle cryo-electron microscopy (cryo-EM) is a technology that allows the observation and the high-resolution 3D structure determination of biomolecules. In this project, the goal is to estimate the angles at which we imaged the 2D projections fro ...

2020

Three-dimensional imaging of integrated circuits with macro- to nanoscale zoom

Gabriel Aeppli, Manuel Guizar Sicairos, Christian David, Michal Odstrcil

The imaging of integrated circuits across different length scales is required for failure analysis, design validation and quality control. At present, such inspection is accomplished using a hierarchy of different probes, from optical microscopy on the mil ...

NATURE PUBLISHING GROUP2019

Inner-Loop-Free ADMM for Cryo-EM

Michaël Unser, Laurène Donati, Emmanuel Emilien Louis Soubies

Thanks to recent advances in signal processing, the interest for fast ℓ1-regularized reconstruction algorithms in cryo-electron microscopy (cryo-EM) has intensified. The approaches based on the alternating-direction of multipliers method (ADMM) are particu ...

IEEE2019

Compressed Sensing for Dose Reduction in STEM Tomography

Michaël Unser, Laurène Donati, Masih Nilchian

We designed a complete acquisition-reconstruction framework to reduce the radiation dosage in 3D scanning transmission electron microscopy (STEM). Projection measurements are acquired by randomly scanning a subset of pixels at every tilt-view (i.e., random ...

IEEE2017

Compressed sensing for STEM tomography

Michaël Unser, Laurène Donati, Masih Nilchian

A central challenge in scanning transmission electron microscopy (STEM) is to reduce the electron radiation dosage required for accurate imaging of 3D biological nano-structures. Methods that permit tomographic reconstruction from a reduced number of STEM ...

2017

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The ability to obtain three-dimensional (3-D) information about morphologies of nanostructures elucidates many interesting properties of materials in both physical and biological sciences. Here we demonstrate a novel method in scanning transmission electro ...

2017

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Deriving accurate three-dimensional (3-D) structural information of materials at the nanometre level is often crucial for understanding their properties. Tomography in transmission electron microscopy (TEM) is a powerful technique that provides such inform ...

Elsevier2017

Shape from bandwidth: the 2-D orthogonal projection case

Martin Vetterli, Benjamin Bejar Haro, Adam James Scholefield

Could bandwidth—one of the most classic concepts in signal processing—have a new purpose? In this paper, we investigate the feasibility of using bandwidth to infer shape from a single image. As a first analysis, we limit our attention to orthographic proje ...

Ieee2017

Three-dimensional electron imaging of dislocations from a single sample tilt

Pascal Fua, Cécile Hébert, Emad Oveisi, Antoine Letouzey

Linear crystal defects called dislocations are one of the most fascinating concepts in materials science that govern mechanical and optoelectronic properties of many materials across a broad range of application. Three-dimensional (3-D) study of dislocatio ...

Wiley2016

Investigation of resins suitable for the preparation of biological sample for 3-D electron microscopy

Giovanni Longo

In the last two decades, the third-dimension has become a focus of attention in electron microscopy to better understand the interactions within subcellular compartments. Initially, transmission electron tomography (TEM tomography) was introduced to image ...

Elsevier2015