Investigation of the neurokinin-1 receptor by fluorescence techniques
Graph Chatbot
Chat with Graph Search
Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.
DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.
The goal of this work was to produce water-soluble fluorescent semiconductor nanocrystals suitable to interact specifically with biomolecules and thereby creating hybrid supramolecular structures composed of nanocrystals and proteins. Hydrophobic TOPO-coat ...
Conformational changes of channel activation: Five enhanced green fluorescent protein (EGFP) mols. (green cylinders) were integrated into the intracellular part of the homopentameric ionotropic 5-HT3 receptor. This allowed the detection of extracellular bi ...
In this thesis, two different fluorescent labeling techniques for in vivo investigations on the 5-HT3 receptor (5-HT3R) functions are presented. This plasma membrane protein contains five subunits surrounding an ion channel that opens after binding of a 5- ...
A new method was developed for the specific and covalent labeling of fusion proteins with small organic probes in vitro and in vivo. The method is based on the construction of fusion proteins of the human O6-alkylguanine-DNA alkyltransferase (hAGT) with a ...
A new technology for the specific and covalent labelling of fusion proteins with small organic molecules in vivo and in vitro has recently been developed in our laboratory. The method is based on the genetically encoded fusion of a target protein to O6-alk ...
Sequential stages in the life cycle of the ionotropic 5-HT3 receptor (5-HT3R) were resolved temporally and spatially in live cells by multicolor fluorescence confocal microscopy. The insertion of the enhanced cyan fluorescent protein into the large intrace ...
Chem. and biol. labeling is fundamental for the elucidation of the function of proteins within biochem. cellular networks. In particular, fluorescent probes allow detection of mol. interactions, mobility and conformational changes of proteins in live cells ...
A combinatorial method for the generation of new split-protein sensors that identifies sites at which a functional domain can be split to give two moieties that can be reconstituted to give a reporter or selectable activity is described. The method is demo ...
(Ecole Polytechnique Federale De Lausanne, Switz.).2005
The authors report on a method for the multicolor imaging of cell surface proteins which is based on the labeling of carrier protein (CP) fusion proteins with different fluorophores. In one application, different generations of a cell surface protein can b ...
How membrane receptors transduce external signals across the membrane and activate intracellular processes is a key question for fundamental research and for therapeutic purposes. Reliable in situ investigations requiest to preserve the full biological act ...