Specification of Replication Techniques, Semi-Passive Replication, and Lazy consensus*
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One of the goals of synthetic biology is the development of an artificial cell. Building an artificial cell from scratch will provide a deeper understanding of fundamental mechanisms and models in biology and promises to contribute towards building novel p ...
Subcellular organization of the bacterial nucleoid and spatiotemporal dynamics of DNA replication and segregation have been studied intensively, but the functional link between these processes remains poorly understood. Here we use quantitative time-lapse ...
Whole cell based screens to identify hits against Mycobacterium tuberculosis (Mtb), carried out under replicating and non-replicating (NRP) conditions, resulted in the identification of multiple, novel but structurally related spiropiperidines with potent ...
Current online applications, such as search engines, social networks, or file sharing services, execute across a distributed network of machines. They provide non-stop services to their users despite failures in the underlying network. To achieve such a hi ...
The emergence of multidrug- and extensively drug-resistant strains of Mycobacterium tuberculosis highlights the need to discover new antitubercular agents. Here we describe the synthesis and characterization of a new series of thienopyrimidine (TP) compoun ...
It is shown that temperature-control led ordered layers of dodecylamine,self-assembled on highly oriented pyrolytic graphite (HOPG), are an appropriate substrate for aligning individual DNA molecules. High resolution atomic force microscopy (AFM) permits v ...
2009
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Replicated systems provide the foundation for most of today’s large-scale services. Engineering such replicated system is an onerous task. The first—and often foremost—step in this task is to establish an appropriate set of design goals, such as availabili ...
2016
Understanding the mechanisms that coordinate replication initiation with subsequent segregation of chromosomes is an important biological problem. Here we report two replication-control mechanisms mediated by a chromosome segregation protein, ParB2, encode ...
Public Library of Science2013
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State machine replication is a technique for making services fault-tolerant by replicating them over a group of machines. Although the theory of state machine replication has been studied extensively, the engineering challenges of converting a theoretical ...
2011
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Telomere shortening can cause detrimental diseases and contribute to aging. It occurs due to the end replication problem in cells lacking telomerase. Furthermore, recent studies revealed that telomere shortening can be attributed to difficulties of the sem ...
