Transduction of CD34+ cells with lentiviral vectors enables the production of large quantities of transgene-expressing immature and mature dendritic cells
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V alpha 14 invariant (V alpha 14i) NKT cells are a subset of regulatory T cells that utilize a semi-invariant TCR to recognize glycolipids associated with monomorphic CD1d molecules. During development in the thymus, CD4(+)CD8(+) V alpha 14i NKT precursors ...
A biological assay was designed and developed to study the effect of excreted morphogens of mechanically stimulated mesenchymal stromal cells (BMSCs) on neighboring cells. Physical contact of different cell pop- ulations is prevented by patterning the cell ...
This unit describes a protocol for the isolation of cells from murine embryonic stem cells with hematopoietic stem cell activity, defined by the ability to reconstitute, long term, multiple lineages of the hematopoietic system of lethally irradiated mice. ...
Embryonic stem (ES) cells represent a unique cellular model to recapitulate in vitro early steps of embryonic development and an unlimited cellular source in therapy for many diseases, as well as targets for drug discovery and toxicology screens. Although ...
The canonical Wnt signaling pathway plays key roles in stem-cell maintenance, progenitor cell expansion, and lineage decisions. Transcriptional responses induced by Wnt depend on the association of either beta-catenin or gamma-catenin with lymphoid enhance ...
Performance degradation data obtained on single solid oxide fuel cells tested at 850°C with air and humidified H2 and using Ni-YSZ anode supported cells are presented. Microscopic investigation is carried out on both anode and cathode to quantify variation ...
Maintenance of the blood system is dependent on dormant haematopoietic stem cells (HSCs) with long-term self-renewal capacity. After injury these cells are induced to proliferate to quickly reestablish homeostasis(1). The signalling molecules promoting the ...
Hair follicles are cutaneous structures characteristic of mammals. These sensitive organs cycle and contain multipotent epithelial stem cells which are implicated in hair growth and hair cycle. A single whisker follicle of the rat hosts up to 1500 stem cel ...
We present the analysis and design of reflecting cells capable of dynamically and independently control the reflection phase of each component of a dual-polarized impinging wave. Such capability is of significant interest for the future implementation of d ...
Continuous turnover of epithelia is ensured by the extensive self-renewal capacity of tissue-specific stem cells. Similarly, epithelial tumour maintenance relies on cancer stem cells (CSCs), which co-opt stem cell properties. For most tumours, the cellular ...
