A number of microfabricated flow cytometry devices have been proposed based on electrical(1,2) or optical(3) detection techniques. On chip marker-free differentiation of cells using impedance spectroscopy was demonstrated between leukocyte sub-populations and on erythrocytes+ghosts mixtures.(4) The detection part of the chip includes 4 microelectrodes patterned on the top and bottom walls of a rectangular microchannel which define two detection volumes (Figure 1). Although preliminary results showed the potential of the impedance measurement using specific frequencies, a systemic approach is required to determine an optimal set of discrimination frequencies for cell sorting.
Haitham Al Hassanieh, Junfeng Guan, Seyedsohrab Madani, Saurabh Gupta, Waleed Ahmed
Mathieu Salzmann, Yinlin Hu, Jingyu Li, Rui Song