Controlled protein adsorption for microimmunoassays
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The present thesis develops some specific aspects of digital holographic microscopy (DHM), namely the effect of shot noise on the phase image accuracy, the use of DHM in micro-tomography and in aberrations evaluation of a microscope objective (MO). DHM is ...
A numerical method for the simulation of the motion of a glacier in two and three dimensions is presented. Glacier ice is treated as an incompressible viscous fluid. The model equations are based on mass, momentum, energy conservation and a specific rheolo ...
In this thesis new imaging approaches for optical microscopy are proposed and studied. They are based on the use of dynamic structured illumination in combination with a demodulation detection concept employing CMOS image detectors. Two particular implemen ...
A numerical model for the adsorption kinetics of proteins on the walls of a microchannel has been developed using the finite element method (FEM) to address the coupling with diffusion phenomena in the restricted microchannel volume. Time evolutions of the ...
With the recent development of fluorescent probes and new high-resolution microscopes, biological imaging has entered a new era and is presently having a profound impact on the way research is being conducted in the life sciences. Biologists have come to d ...
This work addresses two ways of loading proteins on microchannel surfaces for immunoassay applications: the “stop-flow” and the continuous flow processes. The “stop-flow” method consists of successive static incubation periods where the bulk solution deple ...
A method for fabricating sub-micrometer size adhesion sites for future experiments in cell biology is presented. Glass substrates were coated with a thin layer of InSnO and SiO2. The SiO2 was structured by means of electron beam lithography and reactive io ...
Desorption of cadmium (Cd) from sand was studied by both batch and flow-through methods. Batch experiments were conducted at three pH values (5.5, 6.0 and 6.5). In each case, the amount of Cd desorbed was low compared with the quantity of Cd adsorbed previ ...
An algorithm for the simulation of unsteady, viscous, stratified compressible flows, which remains valid at all speeds, is presented. The method is second-order accurate in both space and time and is independent of the Mach number. In order to remove the s ...
Uncontrolled overtopping during flood events can endanger embankment dams. Erosion of the downstream slope and scouring of its base caused by the high velocity and energy of the overflow can indeed lead to breach formation until complete failure. In this c ...