FluorophoreA fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds. Fluorophores are sometimes used alone, as a tracer in fluids, as a dye for staining of certain structures, as a substrate of enzymes, or as a probe or indicator (when its fluorescence is affected by environmental aspects such as polarity or ions).
Optical sectioningOptical sectioning is the process by which a suitably designed microscope can produce clear images of focal planes deep within a thick sample. This is used to reduce the need for thin sectioning using instruments such as the microtome. Many different techniques for optical sectioning are used and several microscopy techniques are specifically designed to improve the quality of optical sectioning. Good optical sectioning, often referred to as good depth or z resolution, is popular in modern microscopy as it allows the three-dimensional reconstruction of a sample from images captured at different focal planes.
Quantitative analysis (chemistry)In analytical chemistry, quantitative analysis is the determination of the absolute or relative abundance (often expressed as a concentration) of one, several or all particular substance(s) present in a sample. Once the presence of certain substances in a sample is known, the study of their absolute or relative abundance could help in determining specific properties. Knowing the composition of a sample is very important, and several ways have been developed to make it possible, like gravimetric and volumetric analysis.
Collimated beamA collimated beam of light or other electromagnetic radiation has parallel rays, and therefore will spread minimally as it propagates. A perfectly collimated light beam, with no divergence, would not disperse with distance. However, diffraction prevents the creation of any such beam. Light can be approximately collimated by a number of processes, for instance by means of a collimator. Perfectly collimated light is sometimes said to be focused at infinity.
Late bindingIn computing, late binding or dynamic linkage—though not an identical process to dynamically linking imported code libraries—is a computer programming mechanism in which the method being called upon an object, or the function being called with arguments, is looked up by name at runtime. In other words, a name is associated with a particular operation or object at runtime, rather than during compilation. The name dynamic binding is sometimes used, but is more commonly used to refer to dynamic scope.
Zodiacal lightThe zodiacal light (also called false dawn when seen before sunrise) is a faint glow of diffuse sunlight scattered by interplanetary dust. Brighter around the Sun, it appears in a particularly dark night sky to extend from the Sun's direction in a roughly triangular shape along the zodiac, and appears with less intensity and visibility along the whole ecliptic as the zodiacal band. Zodiacal light spans the entire sky and contributes to the natural light of a clear and moonless night sky.
Modifiable temporal unit problemThe Modified Temporal Unit Problem (MTUP) is a source of statistical bias that occurs in time series and spatial analysis when using temporal data that has been aggregated into temporal units. In such cases, choosing a temporal unit (e.g., days, months, years) can affect the analysis results and lead to inconsistencies or errors in statistical hypothesis testing. The MTUP is closely related to the modifiable areal unit problem or MAUP, in that they both relate to the scale of analysis and the issue of choosing an appropriate analysis.
Name bindingIn programming languages, name binding is the association of entities (data and/or code) with identifiers. An identifier bound to an object is said to reference that object. Machine languages have no built-in notion of identifiers, but name-object bindings as a service and notation for the programmer is implemented by programming languages. Binding is intimately connected with scoping, as scope determines which names bind to which objects – at which locations in the program code (lexically) and in which one of the possible execution paths (temporally).
SkyglowSkyglow (or sky glow) is the diffuse luminance of the night sky, apart from discrete light sources such as the Moon and visible individual stars. It is a commonly noticed aspect of light pollution. While usually referring to luminance arising from artificial lighting, skyglow may also involve any scattered light seen at night, including natural ones like starlight, zodiacal light, and airglow. In the context of light pollution, skyglow arises from the use of artificial light sources, including electrical (or rarely gas) lighting used for illumination and advertisement and from gas flares.
