Preparation of modified RNA sequences for biological research
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A no. of promising synthetic catalysts for the hydrolytic degrdn. of RNA have been developed in recent years. Some of them show remarkable selectivity for pyrimidine nucleotides. The general problem of all these studies is to distinguish between real effec ...
The reaction of d,l-5'-activated nucleotide of adenosine in the presence and absence of montmorillonite gave 60:40 and 96:4 ratios, resp. of the d,d- and l,l:d,l- and l,d-dimers. [on SciFinder (R)] ...
Pyranosyl-RNA ("p-RNA") is an oligonucleotide system isomeric to natural RNA and composed of the very same building blocks as RNA. Its generational, chem., and informational properties are deemed to be those of an alternative nucleic acid system that cou ...
A review and discussion, with 42 refs. Base pairing in p-RNA (b-D-ribopyranosyl-(4'->2')-oligonucleotides) is not only stronger than in DNA and RNA, but also more selective in the sense that it is strictly confined to the Watson-Crick mode. Homopurine sequ ...
Replication (single-turnover) of pyranosyl-RNA (p-RNA) sequences can be accomplished reliably by template-directed ligation of 2',3'-cyclophosphates of short oligomers. The ligation process was studied using (mostly) octamers as templates and tetramers as ...
A microfluidic device for random fragmentation of DNA or RNA based on the application of a hydrodynamic shear force to these biomolecules is presented. Different from conventional fragmentation instruments, the microchip-based implementation allows molecul ...
A review with 39 refs. Qual. conformational anal. of the entirety of conceivable hexo- and pentopyranosyl oligonucleotide systems derived from the diastereoisomeric aldohexoses (CH2O)6 and aldopentoses (CH2O)5 predicts the existence of a variety of pairing ...
We describe a new procedure to identify RNA or DNA binding sites in proteins, based on a combination of UV cross-linking and single-hit chemical peptide cleavage. Site-directed mutagenesis is used to create a series of mutants with single Asn-Gly sequences ...
The bond lengths and dynamics of intra- and intermol. hydrogen bonds in an RNA kissing complex have been characterized by detg. the NMR relaxation rates of various double- and triple-quantum coherences that involve an imino proton and two neighboring nitro ...
Telomerase is composed of protein and RNA. The RNA serves as a template for telomere DNA synthesis and may also be important for enzyme structure or catalysis. We have used the presence of conserved sequence elements in the promoter and template regions to ...
