The importance of ammonia in mammalian cell culture
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Membrane proteins fulfill many central functions in the biological membrane. The insertion process of these proteins and their structure, which are intimately linked to their function, are not yet well understood. As a model we studied three proteins recon ...
To overcome ammonia inhibition in animal cell culture, a method utilizing porous polytetrafluoroethylene membranes with an assocd. pH gradient was investigated. Model expts. without cells were carried out in three different reactor configurations suitable ...
For the first time, the std. free energy change, DG Deg, of a membrane-inserting protein with a leader sequence has been detd. exptl., using M13 procoat protein as an example. The partition coeff. for the distribution of the procoat protein between the aq. ...
This paper describes the prodn. of IgA antibodies in a range of bioreactor configurations using FCS-contg. RPMI 1640 medium. Under std. batch STR conditions, a max. viable cell and IgA concns. of 9.5*105 cell mL-1 and 47 mg l-1 were obtained. Under fed-bat ...
A chemically-defined protein-free medium (FMX-Turbodoma) has been improved for the production of monoclonal IgA antibodies by hybridoma cells, using a systematic method. Cell growth rate, IgA production, activity and molecular weight pattern have been used ...
A FIA method has been developed and fully characterized for the simultaneous detection of ammonia and glutamine in culture media. Ammonia detection is based upon a chem. method and does not require an electrode. This species diffuses across a hydrophobic p ...
The estn. of the intracellular fluxes of mammalian cells using only the mass balances of the relevant metabolites is not possible because the set of linear equations defined by these mass balances is undetd. Either addnl. exptl. flux data or addnl. theor. ...
The smaller isoform of the GABA synthesizing enzyme glutamic acid decarboxylase, GAD65, is synthesized as a soluble protein that undergoes posttranslational modification(s) in the NH2-terminal region to become anchored to the membrane of small synaptic-lik ...
Bubble-free aeration is applied in animal cell culture processes for oxygenation to avoid shear stress and foaming assocd. with direct sparging into the culture. Oxygen mass transfer across PTFE-membranes was detd. in three different reactor systems that m ...
Monoclonal IgA antibodies have been produced by a hybridoma cell line grown in different bioreactors using serum-contg. and protein-free media in both basal and fortified versions. The effects of culture conditions on IgA prodn. and quality were studied us ...
