Metabolic network analysis of a hybridoma cell line using mass balances and 14C-labeled glucose and glutamine
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The estn. of the intracellular fluxes of mammalian cells using only the mass balances of the relevant metabolites is not possible because the set of linear equations defined by these mass balances is undetd. Either addnl. exptl. flux data or addnl. theor. ...
Continuous cultivation in a glucose-limited chemostat was used to det. the growth parameters of wild-type Bacillus subtilis and of a recombinant, riboflavin-producing strain. Maintenance coeffs. of 0.45 and 0.66 mmol of glucose g-1 h-1 were detd. for the w ...
