Publication

A new method for on-line measurement of the volumetric oxygen uptake rate in membrane aerated animal cell cultures

Abstract

Oxygen is a key substrate in animal cell metab. and its consumption is thus a parameter of great interest for bioprocess monitoring and control. A system for measuring it based on an oxygen balance on the liq. phase was developed. The use of a gas-permeable membrane offered the possibility to provide the required quantity of oxygen into the culture, while avoiding problems of foaming or shear stress generally linked to sparging. This aeration system allowed moreover to keep a known and const. kLa value through cultures up to 400 h. Oxygen uptake rate (OUR) was measured online with a very good accuracy of +-5%, and the specific OUR for a CHO cell line was detd. during batch (growth phase) and continuous culture as, resp., equal to 2.8510-13 and 2.5410-13 mol O2 cell-1 h-1. It was also shown that OUR continuous monitoring gives actually more information about the metabolic state of the culture than the cell concn. itself, esp. during transition phases like the end of the growth phase in a batch culture. [on SciFinder (R)]

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Related concepts (33)
Oxygen
Oxygen is the chemical element with the symbol O and atomic number 8. It is a member of the chalcogen group in the periodic table, a highly reactive nonmetal, and an oxidizing agent that readily forms oxides with most elements as well as with other compounds. Oxygen is Earth's most abundant element, and after hydrogen and helium, it is the third-most abundant element in the universe. At standard temperature and pressure, two atoms of the element bind to form dioxygen, a colorless and odorless diatomic gas with the formula O2.
G1 phase
DISPLAYTITLE:G1 phase The G1 phase, gap 1 phase, or growth 1 phase, is the first of four phases of the cell cycle that takes place in eukaryotic cell division. In this part of interphase, the cell synthesizes mRNA and proteins in preparation for subsequent steps leading to mitosis. G1 phase ends when the cell moves into the S phase of interphase. Around 30 to 40 percent of cell cycle time is spent in the G1 phase.
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