On-line determination of animal cell concentration
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The estn. of the intracellular fluxes of mammalian cells using only the mass balances of the relevant metabolites is not possible because the set of linear equations defined by these mass balances is undetd. Either addnl. exptl. flux data or addnl. theor. ...
The functional role of glutamate decarboxylase (GAD) and its product GABA in pancreatic islets has remained elusive. Mouse β-cells express the larger isoform GAD67, whereas human islets express only the smaller isoform GAD65. We have generated two lines of ...
GLUT2-null mice are hyperglycemic, hypoinsulinemic, hyperglucagonemic, and glycosuric and die within the first 3 weeks of life. Their endocrine pancreas shows a loss of first phase glucose-stimulated insulin secretion (GSIS) and inverse alpha to beta cell ...
Exogenous growth factors normally required in cell culture activate cell proliferation via the mol. controls of cell-cycle progression. Highly differing influences of mitogenic stimulation of Chinese hamster ovary (CHO) cells by insulin and basic fibroblas ...
Animal cell (Chinese Hamster Ovary) concn. was detd. online in a packed bed process using dielec. spectroscopy. This enabled the evaluation of the effect of temp. on specific metabolic rates during 3 mo of continuous culture. The effect of low cultivation ...
Using a specific 13C NMR localization method, 13C label incorporation into the glycogen C1 resonance was measured while infusing [1- 13C]glucose in intact rats. The maximal concentration of [1-13C]glycogen was 5.1 ± 0.6 μmol g-1 (mean ± SE, n = 8). During ...
The amt. of Vitreoscilla Hb (VHb) expression was modulated over a broad range with an isopropyl-beta-D-thiogalactopyranoside- (IPTG)- inducible plasmid, and the consequences on microaerobic Escherichia coli physiol. were examd. in glucose fed-batch cultiva ...
Dielec. spectroscopy was applied to two industrial high cell d. culture processes and used to det. online the concn. of CHO cells immobilized on macroporous microcarriers in a stirred bioreactor and in a packed-bed of disk carriers. The cell concn. predict ...
Continuous cultures of a hybridoma cell line (Zac3) were performed in a stirred tank reactor with protein-free media. At steady state the medium was replaced by one contg. U-14C-labeled glucose. The fluxes leaving the reactor were analyzed with a scintilla ...
Glut-2 is a low-affinity transporter present in the plasma membrane of pancreatic beta-cells, hepatocytes and intestine and kidney absorptive epithelial cells of mice. In beta-cells, Glut-2 has been proposed to be active in the control of glucose-stimulate ...
