Determination of the surface concentration of crown ethers in supported lipid membranes by capacitance measurements
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The secondary structure of alamethicin in lipid membranes below and above the lipid phase transition temp. Tt is detd. by Raman spectroscopy and CD measurements. In both cases structural data are obtained by fitting the exptl. spectra by a superposition of ...
For the first time, the std. free energy change, DG Deg, of a membrane-inserting protein with a leader sequence has been detd. exptl., using M13 procoat protein as an example. The partition coeff. for the distribution of the procoat protein between the aq. ...
Membrane proteins fulfill many central functions in the biological membrane. The insertion process of these proteins and their structure, which are intimately linked to their function, are not yet well understood. As a model we studied three proteins recon ...
A new method is presented for measuring sensitively the interactions between ligands and their membrane-bound receptors in situ by using integrated optics, thus avoiding the need for addnl. labels. Phospholipid bilayers were attached covalently to waveguid ...
In a recent paper, a new class of synthetic lipids, which self-assemble to form monolayers on gold surfaces, was introduced. The addn. of a monolayer of phospholipids to these layers results in robust supported lipid bilayers designed to reconstitute trans ...
Among several effects, ethanol (EtOH) interferes with membrane fluidity and lipid-protein interactions. As proteins are influenced by surrounding lipids, the activity of membrane-bound enzymes such as adenylate cyclase (AC) could be modulated by EtOH, as s ...
Rhodopsin-transducin coupling was used as an assay to investigate a laterally patterned membrane reconstituted with a receptor and its G protein. It served as a model system to show the feasibility to immobilize G protein-coupled receptors on solid support ...
A new simple concept for a miniaturized monolayer trough is described. The overall monolayer area in the expanded state is approx. 150 mm2 and can be reduced by a factor of 2. The surface area is a function of the shape of the meniscus formed by the subpha ...
We demonstrate that the combined application of Langmuir-Blodgett and self-assembly techniques allows the fabrication of patterns with contrasting surface properties on gold substrates. The process is monitored using fluoroscence microscopy and surface pla ...
Raman spectroscopy and x-ray diffraction are used to investigate the influence of surface charges on the structure of ionizable lipid membranes of dimyristoylmethylphosphatidic acid. The membrane surface charge d. is regulated by varying the pH of the aq. ...