Controlled Immobilization of Membrane Proteins to Surfaces for Fourier Transform Infrared Investigations
Graph Chatbot
Chat with Graph Search
Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.
DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.
The nicotinic acetylcholine receptor from Torpedo was immobilised in tethered membranes. Surface plasmon resonance was used to quantify the binding of ligands and antibodies to the receptor. The orientation and structural integrity of the surface-reconstit ...
Many fundamental processes that govern the function of biological cells occur on or immediately under the cell membrane. Fluorescence marking of specific sites has had a large impact in the recognition of these processes. In addition, observation in an app ...
In this work, the specific and nonspecific binding of proteins to planar surfaces was investigated. The surfaces were first modified by the spontaneous adsorption of a number of synthesized 1,2-diacyl-sn-glycero-3-phosphatidylcholine lipids with different ...
Membrane proteins fulfill many central functions in the biological membrane. The insertion process of these proteins and their structure, which are intimately linked to their function, are not yet well understood. As a model we studied three proteins recon ...
For the first time, the std. free energy change, DG Deg, of a membrane-inserting protein with a leader sequence has been detd. exptl., using M13 procoat protein as an example. The partition coeff. for the distribution of the procoat protein between the aq. ...
The formation of supported lipid mono- and bilayers on a thiol-coated surface was monitored by utilising the acoustic Love waveguide device. The detection of the lipid layers was used as a model system for relating the phase response to the deposited mass ...
A general method for understanding the mechanisms of ligand recognition and activation of G protein-coupled receptors has been developed. A study of ligand-receptor interactions in the prototypic seven-transmembrane neurokinin-2 receptor (NK2) using this f ...
A lipopeptide, carrying the antigenic peptide segment 135-154 of VP1, one of the capsid proteins of the picornavirus which causes foot-and-mouth disease in cattle, was investigated in lipid monolayers on the water surface and on hydrophobic solid supports. ...
In a recent paper, a new class of synthetic lipids, which self-assemble to form monolayers on gold surfaces, was introduced. The addn. of a monolayer of phospholipids to these layers results in robust supported lipid bilayers designed to reconstitute trans ...
A bilayer lipid membrane sensor comprises (1) a gold recording surface; (2) a 1st lipid layer which is an imperfect layer of a thiolipid comprising the 2 phospholipid mols. linked to each end of a disulfide group, each through an oxyethylene chain short en ...
