Publication

Production of lactate using crabtree negative organisms expressing recombinant lactate dehydrogenase genes

Related publications (38)

Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity

Florian Maria Wurm, Martin Jordan, Mathieu Benjamin Stettler, Francesca Zagari

Lactate production is monitored in industrial processes as a crucial metabolite for cultured mammalian cells. Typically lactate is strongly produced during the exponential growth phase, while its net consumption is frequently observed when cells enter into ...

Elsevier2013

High expression of the aspartate–glutamate carrier Aralar1 favors lactate consumption in CHO cell culture

Florian Maria Wurm, Lucia Baldi Unser, Martin Jordan, Mathieu Benjamin Stettler, Francesca Zagari

Process performance of mammalian cell cultures can be strongly impacted by high lactate accumulation, which can be a clone or media-dependent characteristic. In this study, the expression of specific genes was measured in several Chinese hamster ovary cell ...

2013

The Presence of Ultralow Densities of Nanocrystallites in Amorphous Poly(lactic acid) Microspheres

Sylvie Roke, Hilton Barbosa De Aguiar

Ultralow densities of crystalline nanospheres have been detected in amorphous polymer microspheres by utilizing the unique sensitivity of second-order nonlinear optical techniques to anisotropy. Vibrational sum frequency scattering (SFS) and X-ray diffract ...

Amer Chemical Soc2013

Multidisciplinary Analysis of the Metabolic Shift to Lactate Consumption in CHO Cell Culture

Francesca Zagari

Mammalian cells represent the most widely used host system for the industrial production of recombinant therapeutic proteins. In order to increase productivity, chemically defined media are often used and optimized to provide the cells with the necessary n ...

EPFL2012

Cell metabolism monitoring using a microfluidic cartridge with enzyme-based microreactors

Nico de Rooij, Peter Van der Wal, Sara Talaei

We present a novel microfluidic approach for on-line analysis of cell metabolism by simultaneous measurement of two important indicators (glucose and lactate) in a small volume (100 µl) of cell-culture medium. The in-vitro study of cell metabolism is espec ...

2012

Monitoring system for cell culture

Yann Barrandon, Eric Meurville, Georges Abou Jaoudé

Cell culture environment monitoring system (6) for monitoring parameters relevant to cell growth in at least one culture dish (4) containing a cell growth medium (14), including at least one sensing device (22, 22') configured to measure environmental para ...

2012

Highly Sensitive Carbon Nanotube-Based Sensing for Lactate and Glucose Monitoring in Cell Culture

Giovanni De Micheli, Sandro Carrara, Cristina Boero, Giovanna Del Vecchio

Monitoring of metabolic compounds in cell cultures can provide real-time information of cell line status. This is particularly important in those lines not fully known, as the case of embryonic and mesenchymal cells. On the other hand, such approach can pa ...

Institute of Electrical and Electronics Engineers2011

Lentiviral Transgenesis as a Tool to Study the Role of Uncoupling Protein Isoforms in the Nervous System

Hélène Danielle Perreten Lambert

A tight coupling exists between synaptic activity and glucose utilization by astrocytes. Metabolic cooperation between neurons and astrocytes mediates this coupling. During synaptic activation, glutamate that is released in the synaptic cleft as a neurotra ...

EPFL2011

OrbShake Bioreactors for Mammalian Cell Cultures

Stéphanie Tissot

Most of the biopharmaceuticals nowadays are produced by animal cells. The worldwide demand for a specific group of biopharmaceuticals, the monoclonal antibodies (mAbs), has significantly increased this last decade. The mAbs are used as therapeutics in part ...

EPFL2011

Fuel bioethanol production from whey permeate

François-Nicolas de Glutz

The objective of this thesis was to evaluate the feasibility for the production of fuel bioethanol from whey permeate. The identification of the limiting factors of the process revealed that the stabilization of the substrate was critical to the use of whe ...

EPFL2009

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