Metabolic engineering under uncertainty-II: Analysis of yeast metabolism
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The invention provides methods and materials related to the prodn. of org. products. Specifically, the invention provides various recombinant yeast cells, methods for culturing yeast cells, methods for making yeast cells, nucleic acid constructs, and metho ...
In wild-type budding yeast strains, the proteins encoded by SIR3, SIR4 and RAP1 co-localize with telomeric DNA in a limited number of foci in interphase nuclei. Immunostaining of Sir2p shows that in addition to a punctate staining that coincides with Rap1 ...
A prior genetic study indicated that activity of Sir silencing proteins at a hypothetical AGE locus is essential for long life span. In this model, the SIR4-42 mutation would direct the Sir protein complex to the AGE locus, giving rise to a long life span. ...
A simple definition of the energetic growth efficiency both in terms of enthalpy and free energy is given and discussed. In cultures growing by respiratory catabolism, these efficiencies may conveniently be obsd. online by isothermal reaction calorimetry. ...
This paper describes an improvement of a method for continuous calibration of the global heat transfer coeff., recently described in literature [A. Tietze, Moglichkeiten und Grenzen der Temperaturschwingungskalorimetrie, Doctoral Thesis, Technishe Universi ...
Animal cell (Chinese Hamster Ovary) concn. was detd. online in a packed bed process using dielec. spectroscopy. This enabled the evaluation of the effect of temp. on specific metabolic rates during 3 mo of continuous culture. The effect of low cultivation ...
Chemostat cultures of an aerobic fermenting yeast (Saccharomyces cerevisiae) and an aerobic respiring yeast (Kluyveromyces fragilis) were grown under dual C + N limitation in an isothermal reaction calorimeter. The dual limitations resulted in uncoupled ox ...
The dynamic behavior of a continuous culture of Saccharomyces cerevisiae subjected to a sudden increase in the diln. rate has been successfully modelled for anaerobic growth on glucose, and for aerobic growth on acetate, on ethanol, and on glucose. The cat ...
The bioconversion of L-phenylalanine (L-Phe) to 2-phenylethanol (PEA) by the yeast Saccharomyces cerevisiae is limited by the toxicity of the product. PEA extn. by a sep. org. phase in the fermenter is the ideal in situ product recovery (ISPR) technique to ...
Continuous cultures of a hybridoma cell line (Zac3) were performed with 2 14C-labeled substrates: glucose and glutamine. A simplified network was used to describe cell metab. This simple network gave a good idea of the main fluxes into the cell, esp. for c ...
