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BACKGROUND: Modulation of microtubule dynamics is crucial for proper cell division. While a large body of work has made important contributions to our understanding of the mechanisms governing microtubule dynamics in vitro, much remains to be learned about ...
We present an atomic force microscope ~AFM for operation at low temperatures under ultrahigh vacuum conditions. It uses the laser beam deflection method to measure the bending of the cantilever. The four quadrant photodiode allows the detection of vertical ...
Specimen charging under X-ray illumination is a well known phenomenon that can seriously obstruct the analysis of insulating samples. Synchrotron X-PEEM spectromicroscopy can reach a lateral resolution of 20 nm, 1-2 orders of magnitude larger than electron ...
The fabrication and application of photoplastic scanning force microscopy (SFM) probes has been demonstrated. Simple molding and replication techniques, inexpensive photoplastic material, reusable molds and several layers per probe make it a very attractiv ...
Structural information on membrane proteins is sparse, yet they represent an important class of proteins that is encoded by about 30% of all genes. Progress has primarily been achieved with bacterial proteins, but efforts to solve the structure of eukaryot ...
To study bone adaptation to detraining in growing rats, nine weeks-old immature female Wistar rats (n=110) were subjected to treadmill running programs (30 or 60 minutes-a-day) for up to 15 weeks, followed by unrestricted cage activities for the subsequent ...
The mechanisms governing centrosome duplication remain poorly understood. We identified a gene called sas-4 that is essential for this process in C. elegans. SAS-4 encodes a predicted coiled-coil protein that localizes to a tiny dot in the center of centro ...
SU-8 is a photoplastic polymer with a wide range of applications in microtechnology. Cantilevers designed for a commercial Atomic Force Microscope (AFM) were fabricated in SU-8. The mechanical properties of these cantilevers were investigated using two mic ...
gamma-Tubulin-containing complexes are thought to nucleate and anchor centrosomal microtubules (MTs). Surprisingly, a recent study (Strome, S., J. Powers, M. Dunn, K. Reese, C.J. Malone, J. White, G. Seydoux, and W. Saxton. Mol. Biol. Cell. 12:1751-1764) s ...
Many fundamental processes that govern the function of biological cells occur on or immediately under the cell membrane. Fluorescence marking of specific sites has had a large impact in the recognition of these processes. In addition, observation in an app ...
