Catégorie
Glycobiologie
Publications associées (85)
Identification of N-glycan positional isomers by combining IMS and vibrational fingerprinting of structurally determinant CID fragments
Thomas Rizzo, Ahmed Ben Faleh, Stephan Warnke, Priyanka Bansal
While glycans are present on the surface of cells in all living organisms and play key roles in most biological processes, their isomeric complexity makes their structural characterization challenging. Of particular importance are positional isomers, for w ...
ROYAL SOC CHEMISTRY2022Combining multi-stage ion mobility spectrometry with cryogenic infrared spectroscopy for glycan analysis
Structural analysis of biomolecules is essential to understanding biological processes. Among the various classes of biomolecules, glycans have gained significant attention recently due to their ubiquitous roles in biological systems. However, study of gly ...
EPFL2022Identification of Mobility-Resolved N-Glycan Isomers
Thomas Rizzo, Robert Paul Pellegrinelli, Ahmed Ben Faleh, Stephan Warnke, Priyanka Bansal
ABSTRACT: Glycan analysis has evolved considerably during the last decade. The advent of high-resolution ion-mobility spectrometry has enabled the separation of isomers with only the slightest of structural differences. However, the ability to separate suc ...
AMER CHEMICAL SOC2022A new approach for identifying positional isomers of glycans cleaved from monoclonal antibodies
Thomas Rizzo, Ahmed Ben Faleh, Stephan Warnke, Priyanka Bansal, Vasyl Yatsyna, Natalia Yalovenko
Glycosylation patterns in monoclonal antibodies (mAbs) can vary significantly between different host cell types, and these differences may affect mAbs safety, efficacy, and immunogenicity. Recent studies have demonstrated that glycan isomers with the termi ...
ROYAL SOC CHEMISTRY2021Analyzing glycans cleaved from a biotherapeutic protein using a combination of ion mobility spectrometry and cryogenic ion spectroscopy
The characterization of biomolecules plays a central role in biomedical research. The development of state-of-the-art technologies is needed to make such analyses faster, more sensitive, and more accurate. While mass spectrometry continues to play a centra ...
EPFL2021A gas phase approach for glycan analysis: Combining ultrahigh-resolution ion mobility spectrometry with cryogenic vibrational spectroscopy
The last decades have been punctuated by the development of revolutionary analytical technologies that have enabled the analysis of biomolecular structures and unraveled the roles they play in biological systems. However, certain classes of biomolecules re ...
EPFL2021GRASP55 regulates intra-Golgi localization of glycosylation enzymes to control glycosphingolipid biosynthesis
Giovanni D'Angelo, Charlotte Julie Caroline Gehin, Laura Capolupo, Riccardo Rizzo
The Golgi apparatus, the main glycosylation station of the cell, consists of a stack of discontinuous cisternae. Glycosylation enzymes are usually concentrated in one or two specific cisternae along the cis-trans axis of the organelle. How such compartment ...
WILEY2021Combining cryognic ion spectroscopy with ion mobility for the study of glycan fragmentation
The importance of glycans in biological processes are matched by their structural complexity. Coating the surfaceof most living cells, glycans play key roles in many biological processes, and the role they play is closely relatedto their structures. Struct ...
EPFL2021Identification of Allobaculum mucolyticum as a novel human intestinal mucin degrader
The human gut microbiota plays a central role in intestinal health and disease. Yet, many of its bacterial constituents are functionally still largely unexplored. A crucial prerequisite for bacterial survival and proliferation is the creation and/or exploi ...
TAYLOR & FRANCIS INC2021The Mammalian Membrane Microenvironment Regulates the Sequential Attachment of Bacteria to Host Cells
Alexandre Louis André Persat, Xavier Jean-Yves Pierrat
Pathogen attachment to host tissue is critical in the progress of many infections. Bacteria use adhesion in vivo to stabilize colonization and subsequently regulate the deployment of contact-dependent virulence traits. To specifically target host cells, th ...
2021