Publications associées à Cal (culture in vitro)

Preexisting variation in DNA damage response predicts the fate of single mycobacteria under stress

John McKinney, Neeraj Dhar, Giulia Manina, Lalit Kumar Singh

Clonal microbial populations are inherently heterogeneous, and this diversification is often considered as an adaptation strategy. In clinical infections, phenotypic diversity is found to be associated with drug tolerance, which in turn could evolve into g ...

2019

Effect of mechanical damage and wound healing on the viscoelastic properties of stems of flax cultivars (Linum usitatissimum L. cv. Eden and cv. Drakkar)

Véronique Michaud, Sara Dalle Vacche, Federica Sordo

As plant fibres are increasingly used in technical textiles and their composites, underlying principles of wound healing in living plant fibres are relevant to product quality, and provide inspiration for biomimetic healing in synthetic materials. In this ...

Public Library of Science2017

Advanced technologies to control stem-cell based organogenesis

Nathalie Brandenberg

Organoids represent the first in vitro cell culture systems that closely resemble native tissues in terms of cellular composition, architecture and key aspects of physiology. Through the discovery of these cultures, researchers could demonstrate that stem ...

EPFL2017

Plasmonic nanohole array biosensor for label-free and real-time analysis of live cell secretion

Hatice Altug, Xiaokang Li, Filiz Yesilköy, Alexander Belushkin, Maria Soler Aznar, Cenk Ibrahim Özdemir

Cell secretion dynamics plays a central role in physiological and disease processes. Due to its various temporal profiles, it is essential to implement a precise detection scheme for continuous monitoring of secretion in real time. The current fluorescent ...

Royal Society of Chemistry2017

Silver nanoparticles inhibit fish gill cell proliferation in protein-free culture medium

Kristin Schirmer, Laura Sigg, Renata Behra, Yang Yue

While short-term exposures of vertebrate cells, such as from fish, can be performed in defined, serum-free media, long-term cultures generally require addition of growth factors and proteins, normally supplied with a serum supplement. However, proteins are ...

Taylor & Francis Ltd2016

Dielectric Elastomer Actuator for Mechanical Loading of 2D Cell Cultures

Herbert Shea, Samuel Rosset, Alexandre Poulin

We demonstrate the use of dielectric elastomer actuators (DEAs) for mechanical stimulation of cells in vitro. The development of living tissues is regulated by their mechanical environment through the modification of fundamental cellular functions such as ...

Royal Society of Chemistry2016

Miniaturized Dielectric Elastomer Actuator for Mechanical Stimulation of Monolayer Cell Cultures

Alexandre Poulin

This thesis advances the field of dielectric elastomer actuators (DEAs) through the development of device designs, fabrication processes, strain characterization technique and modelling tool. It provides the first demonstration that DEAs can be interfaced ...

EPFL2016

Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Xiao Shen, Dominique Terry Monteil Jr, Giulia Tontodonati

Disposable orbitally shaken TubeSpin bioreactor 600 tubes (TS600s) were recently developed for the bench-scale cultivation of animal cells in suspension. Here we compared batch cultures of Sf9 insect cells in TS600s, spinner flasks, and shake flasks. Super ...

Elsevier2016

Differential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles

Therapeutic engineered nanoparticles (NPs), including ultrasmall superparamagnetic iron oxide (USPIO) NPs, may accumulate in the lower digestive tract following ingestion or injection. In order to evaluate the reaction of human colon cells to USPIO NPs, th ...

Dove Medical Press Ltd2014

Silk Hydrogels as Soft Substrates for Neural Tissue Engineering

Jeffrey Alan Hubbell, Federico Tortelli, Amy Marie Hopkins

There is great need for soft biomaterials that match the stiffness of human tissues for tissue engineering and regeneration. Hydrogels are frequently employed for extracellular matrix functionalization and to provide appropriate mechanical cues. It is chal ...

Wiley-V C H Verlag Gmbh2013