Concept

Cal (culture in vitro)

Publications associées (33)

Gene Delivery with Hyperbranched Polylysine

The delivery of exogenous nucleic acids into mammalian cells is a valuable technique for basic research studies on the expression, regulation and function of genes and proteins. At the same time, gene delivery to cultivated mammalian cells has become funda ...

EPFL2011

Self-organization is a dynamic and lineage-intrinsic property of mammary epithelial cells

Léa Chanson

Loss of organization is a principle feature of cancers; therefore it is important to understand how normal adult multilineage tissues, such as bilayered secretory epithelia, establish and maintain their architectures. The self-organization process that dri ...

2011

Fully differential low-noise amplifier with offset reduction for high-resolution neural signal recording

Pierre-André Farine, Steve Tanner, Urs Alexander Müller

A prototype of a high-density multielectrode array for in vitro recording of electrogenic cell networks has been developed. On a surface of 1.92x1.92mm2, it includes 32x32 pixels with a dimension of 60x60µm2. For local amplification of the sensed extracell ...

2010

Carbon Nanotubes-Based Electrochemical Sensing for Cell Culture Monitoring

Giovanni De Micheli, Sandro Carrara, Cristina Boero, Giovanna Del Vecchio

Monitoring of metabolic compounds, such as glucose and lactate, is extensively reported in literature, especially for clinical purposes. Instead, the application of such technologies for monitoring metabolites in cell cultures has not been explored. From o ...

2010

Induction of A9 dopaminergic neurons from neural stem cells improves motor function in an animal model of Parkinsons disease

Romain Zufferey

Neural stem cells (NSCs) are widely endorsed as a cell source for replacement strategies in neurodegenerative disease. However, their usefulness is currently limited by the inability to induce specific neurotransmitter phenotypes in these cells. In order t ...

Oxford University Press2008

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Florian Maria Wurm, Lucia Baldi Unser, Natalie Muller, Philippe Pierre Girard

Recent advances in genomics, proteomics, and structural biology raised the general need for significant amounts of pure recombinant protein (r-protein). Because of the difficulty in obtaining in some cases proper protein folding in bacteria, several method ...

2005

The Listeria monocytogenes lemA gene product is not required for intracellular infection or to activate fMIGWII-specific T cells

Clearance of the intracellular bacterial pathogen Listeria monocytogenes requires antigen-specific CD8(+) T cells. Recently it was shown that activation of class Ib major histocompatibility complex (MHC)-restricted CD8(+) T cells alone is sufficient for im ...

American Society for Microbiology2003

Spatial control of neuronal cell attachment and differentiation on covalently patterned laminin oligopeptide substrates

Patrick Aebischer

The spatial control of neuronal cell attachment and differentiation via specific receptor mediated interactions, may provide an effective means for the in vitro reconstruction of neuronal cell architecture. In this study, receptor-specific oligopeptide seq ...

1994

Developmental extinction of liver lipoprotein lipase mRNA expression might be regulated by an NF-1-like site

Johan Auwerx, Kristina Schoonjans

The molecular mechanism underlying the extinction of lipoprotein lipase (LPL) expression in rat liver during development was investigated. A mouse (BWTG3) and a rat (7777) hepatoma, both of which exhibit characteristics of fetal hepatocytes, were found to ...

1993

Effect of a near-critical and supercritical fluid on the viability ratio of microbial cells

Urs von Stockar, Ian William Marison

The following paper describes how pressurized CO2 under near-crit. and supercrit. conditions affects the survival of a range of yeast and bacterial cells. Kluyveromyces fragilis was found to be the most resistant of the test organisms studied with relative ...

Elsevier1992

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