Concept

Gene targeting

Publications associées (101)

Characterization and modeling of a ZnO nanowire ultraviolet photodetector with graphene transparent contact

We report the demonstration of a ZnO nanowire ultraviolet photodetector with a top transparent electrode made of a few-layered graphene sheet. The nanowires have been synthesized using a low-cost electrodeposition method. The detector is shown to be visibl ...

American Institute of Physics2013

Impact of copy number variations (CNVs) on long-range gene regulation at the HoxD locus

Denis Duboule

Copy number variations are genomic structural variants that are frequently associated with human diseases. Among these copy number variations, duplications of DNA segments are often assumed to lead to dosage effects by increasing the copy number of either ...

Natl Acad Sciences2012

Circadian Dbp Transcription Relies on Highly Dynamic BMAL1-CLOCK Interaction with E Boxes and Requires the Proteasome

Felix Naef, David Michael Suter

The transcription factors BMAL1 and CLOCK drive the circadian transcription of clock and clock-controlled genes, such as Dbp. To investigate the kinetics of BMAL1 binding to target genes in real time, we generated a cell line harboring tandem arrays of Dbp ...

Elsevier2012

Spatially and genetically distinct control of seed germination by phytochromes A and B

Christian Fankhauser

Phytochromes phyB and phyA mediate a remarkable developmental switch whereby, early upon seed imbibition, canopy light prevents phyB-dependent germination, whereas, later on, it stimulates phyA-dependent germination. Using a seed coat bedding assay where t ...

Cold Spring Harbor Lab Press, Publications Dept2012

Variegated gene expression caused by cell-specific long-range DNA interactions

Daniël Noordermeer

Mammalian genomes contain numerous regulatory DNA sites with unknown target genes. We used mice with an extra β-globin locus control region (LCR) to investigate how a regulator searches the genome for target genes. We find that the LCR samples a restricted ...

2011

ZFN-Site searches genomes for zinc finger nuclease target sites and off-target sites

Giovanna Ambrosini, Christian Iseli, Philipp Bucher

Background: Zinc Finger Nucleases (ZFNs) are man-made restriction enzymes useful for manipulating genomes by cleaving target DNA sequences. ZFNs allow therapeutic gene correction or creation of genetically modified model organisms. ZFN specificity is not a ...

BioMed Central2011

GETPrime: a gene- or transcript-specific primer database for quantitative real-time PCR

Bart Deplancke, Jacques Rougemont, Fabrice David, Andreas Massouras, Alexandre Gattiker, Frederik Decouttere

The vast majority of genes in humans and other organisms undergo alternative splicing, yet the biological function of splice variants is still very poorly understood in large part because of the lack of simple tools that can map the expression profiles and ...

2011

Reshuffling genomic landscapes to study the regulatory evolution of Hox gene clusters

Denis Duboule

The emergence of Vertebrata was accompanied by two rounds of whole-genome duplications. This enabled paralogous genes to acquire novel functions with high evolutionary potential, a process suggested to occur mostly by changes in gene regulation, rather tha ...

2011

Evaluation of potential reference genes for reverse transcription-qPCR studies of physiological responses in Drosophila melanogaster

Drosophila melanogaster is one of the most important genetic models and techniques such as reverse transcription quantitative real-time PCR (RT-qPCR) are being employed extensively for deciphering the genetics basis of physiological functions. In RT-qPCR, ...

Elsevier2011

Life cycle studies of the hexose transporter of Plasmodium species and genetic validation of their essentiality

Summary A Plasmodium falciparum hexose transporter (PfHT) has previously been shown to be a facilitative glucose and fructose transporter. Its expression in Xenopus laevis oocytes and the use of a glucose analogue inhibitor permitted chemical validation of ...

Wiley-Blackwell2010

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