Concept
Ultracentrifugeuse
Publications associées (30)
Single-Chain Nanoparticles for Intracellular Drug Delivery
Single-chain nanoparticles (SCNPs) are a type of polymeric nanoparticles formed by collapsing/folding individual polymer chains via intra-molecular interactions. Their emergence holds promise to construct sub-20 nm polymeric nanoparticles with defined stru ...
EPFL2022Control and Characterization of the Compactness of Single-Chain Nanoparticles
Francesco Stellacci, Luciano Andres Abriata, Lixia Wei, Suiyang Liao
Polymers when self-cross-linked into single-chain nanoparticles bear some resemblance to folded proteins; yet proteins have clear energy landscapes that determine precisely folded structures, while single-chain polymer nanoparticles (SCNPs) have more undef ...
AMER CHEMICAL SOC2021A simple micropreparative gel electrophoresis technique for purification of proteins, nucleic acids, and bioconjugates
Ardemis Anoush Boghossian, Sayyed Hashem Sajjadi, Shang-Jung Wu, Vitalijs Zubkovs
The biochemical and biomedical fields hinge on the ability to effectively separate and purify biological macromolecules. Though this need is largely addressed with a variety of chromatographic and electrophoretic purification techniques, such techniques ar ...
2021An Instrumented High-Speed Rotor with Embedded Telemetry for The Continuous Spatial Pressure Profile Measurement in Gas Lubricated Bearings: A Proof of Concept
Jürg Alexander Schiffmann, Karim Magdi Zakaria Abdelrahman Shalash
Pressure is one of the constitutional quantities governing the flow field in gas lubricated bearings. Knowledge of the pressure is of principal importance in the fundamental understanding of such bearings and for the calibration of reduced order models. Th ...
2020An Instrumented High-Speed Rotor With Embedded Telemetry For The Continuous Spatial Pressure Profile Measurement In Gas Lubricated Bearings: A Proof Of Concept
Jürg Alexander Schiffmann, Karim Magdi Zakaria Abdelrahman Shalash
Gas lubricated bearings are capable of supporting high speed rotors with satisfactory load capacities. Pressure is one of the constitutional quantities governing the flow field in gas lubricated bearings. Knowledge of the pressure is of principal importanc ...
Amer Soc Mechanical Engineers2019On the effect of ligand shell heterogeneity on nanoparticle/protein binding thermodynamics
Francesco Stellacci, Ahmet Bekdemir, Suiyang Liao
Nonspecific protein adhesion to nanoparticle (NP) has been proven to have important implications in nanomedicine. However, there are only a few examples of careful studies relating protein binding thermodynamics to NP physicochemical features. In particula ...
2019Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes
Florian Maria Wurm, David Hacker, Xiao Shen, Dominique Terry Monteil Jr, Likuan Zhu
Orbitally shaken cylindrical bioreactors [OrbShake bioreactors (OSRs)] without an impeller or sparger are increasingly being used for the suspension cultivation of mammalian cells. Among small volume OSRs, 50-mL tubes with a ventilated cap (OSR50), origina ...
American Chemical Society2017A centrifugation-based physicochemical characterization method for the interaction between proteins and nanoparticles
Francesco Stellacci, Ahmet Bekdemir
Nanomedicine requires in-depth knowledge of nanoparticle-protein interactions. These interactions are studied with methods limited to large or fluorescently labelled nanoparticles as they rely on scattering or fluorescence-correlation signals. Here, we hav ...
Nature Publishing Group2016Push-pull dioxaborine as fluorescent molecular rotor: far-red fluorogenic probe for ligand-receptor interactions
Fluorescent solvatochromic dyes and molecular rotors have attracted considerable attention as fluorogenic probes because of background-free detection of biomolecules in live cells in no-wash conditions. Herein, we introduce a push-pull boron-containing (di ...
Royal Soc Chemistry2016A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation
Jutta Christine Wandrey, Ahmet Bekdemir, Helmut Coelfen
Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose of this ...
Public Library of Science2015