Concept

Protein splicing

Publications associées (38)

Mutant Exon1 Huntingtin Aggregation is Regulated by T3 Phosphorylation-Induced Structural Changes and Crosstalk between T3 Phosphorylation and Acetylation at K6

Giovanni Dietler, Hilal Lashuel, Francesco Simone Ruggeri, Anass Chiki, Sean Michael Deguire, Urszula Beata Cendrowska, Annalisa Ansaloni, Ritwik Burai, Sophie Vieweg

Herein, we used protein semisynthesis to investigate, for the first time, the effect of lysine acetylation and phosphorylation, as well as the crosstalk between these modifications on the structure and aggregation of mutant huntingtin exon1 (Httex1). Our r ...

Wiley-Blackwell2017

Impact of polymer-modified gold nanoparticles on brain endothelial cells: exclusion of endoplasmic reticulum stress as a potential risk factor

Harm-Anton Klok, Matthew Gibson

A library of polymer-coated gold nanoparticles (AuNPs) differing in size and surface modifications was examined for uptake and induction of cellular stress responses in the endoplasmic reticulum (ER stress) in human brain endothelial cells (hCMEC/D3). ER s ...

Informa Healthcare2016

Elucidating the Role of Site-Specific Nitration of alpha-Synuclein in the Pathogenesis of Parkinson's Disease via Protein Semisynthesis and Mutagenesis

Hilal Lashuel, Nadine Aït Bouziad, Anass Chiki, Ritwik Burai

Parkinsons disease (PD) is characterized by the loss of dopaminergic neurons in the substantia nigra and the presence of intraneuronal inclusions consisting of aggregated and post-translationally modified a-synuclein (a-syn). Despite advances in the chemic ...

Amer Chemical Soc2015

Semisynthesis and Enzymatic Preparation of Post-translationally Modified α-Synuclein

Hilal Lashuel, Bruno Claude Daniel Fauvet

Posttranslational modifications (PTMs) serve as molecular switches for regulating protein folding, function, and interactome and have been implicated in the misfolding and amyloid formation by several proteins linked to neurodegenerative diseases, includin ...

2015

Imaging and manipulating proteins in live cells through covalent labeling

Kai Johnsson, Lin Xue, Julien Hiblot, Iuliia Karpenko

The past 20 years have witnessed the advent of numerous technologies to specifically and covalently label proteins in cellulo and in vivo with synthetic probes. These technologies range from self-labeling proteins tags to non-natural amino acids, and the q ...

Nature Publishing Group2015

Differential dependence on N-glycosylation of anthrax toxin receptors CMG2 and TEM8

Françoise Gisou van der Goot Grunberg, Julie Deuquet Ariosa

ANTXR 1 and 2, also known as TEM8 and CMG2, are two type I membrane proteins, which have been extensively studied for their role as anthrax toxin receptors, but with a still elusive physiological function. Here we have analyzed the importance of N-glycosyl ...

Public Library of Science2015

Fluorescent Labeling of SNAP-Tagged Proteins in Cells

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius

One of the most prominent self-labeling tags is SNAP-tag. It is an in vitro evolution product of the human DNA repair protein O6 -alkylguanine-DNA alkyltransferase (hAGT) that reacts specifically with benzylguanine (BG) and benzylchloropyrimidine (CP) deri ...

Springer2015

One-Pot Semisynthesis of Exon 1 of the Huntingtin Protein: New Tools for Elucidating the Role of Posttranslational Modifications in the Pathogenesis of Huntington's Disease

Giovanni Dietler, Hilal Lashuel, Francesco Simone Ruggeri, Annalisa Ansaloni, Jae Sun Jeong

The natural enzymes involved in regulating many of the posttranslational modifications (PTMs) within the first 17 residues (Nt17) of Huntingtin exon1 (Httex1) remain unknown. A semisynthetic strategy that allows the site-specific introduction of PTMs withi ...

Wiley-Blackwell2014

Fluorescent Labeling of SNAP-Tagged Proteins in Cells

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius

One of the most prominent self-labeling tags is SNAP-tag. It is an in vitro evolution product of the human DNA repair protein O 6-alkylguanine-DNA alkyltransferase (hAGT) that reacts specifically with benzylguanine (BG) and benzylchloropyrimidine (CP) deri ...

Springer New York2014

A chemostat array enables the spatio-temporal analysis of the yeast proteome

Michaël Unser, Felix Naef, Sebastian Maerkl, Ricard Delgado Gonzalo, Nicolas Dénervaud, Arun Stephen Rajkumar, Johannes Becker

Observing cellular responses to perturbations is central to generating and testing hypotheses in biology. We developed a massively parallel microchemostat array capable of growing and observing 1,152 yeast-GFP strains on the single-cell level with 20 min t ...

National Academy of Sciences2013

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