Concept
Α-amylase
Publications associées (8)
Difference in astringency of the main pea protein fractions
Francesco Stellacci, Hanna Fanny Clarisse Lesme
Interactions between food and saliva govern complex mouthfeel perceptions such as astringency. Herein, we present a study of the interactions of salivary proteins with the main pea protein fractions that are obtained by isoelectric and salt precipitation ( ...
London2023Pancreatic α- and β-Cellular Clocks Have Distinct Molecular Properties and Impact on Islet Hormone Secretion and Gene Expression
Michaël Unser, Daniel Sage, Cédric Gobet, Frédéric Bruno Martin Gachon
A critical role of circadian oscillators in orchestrating insulin secretion and islet gene transcription has been demonstrated recently. However, these studies focused on whole islets and did not explore the interplay between α-cell and β-cell clocks. We p ...
2017Establishing the Catalytic Mechanism of Human Pancreatic α-Amylase with QM/MM Methods
In this work, we studied the catalytic mechanism of human pancreatic α-amylase (HPA). Our goal was to determine the catalytic mechanism of HPA with atomic detail using computational methods. We demonstrated that the HPA catalytic mechanism consists of two ...
2015Switch-peptides: controlling biological function and self-assembly of amyloid ß-derived peptides using enzyme-triggered acyl migrations
Conformational transitions of peptides and proteins have recently moved to the center of interest in various domains of research at the interface of chemistry, biology, and medicine due to their implication in an increasing number of diseases in which the ...
EPFL2005Transfer and disproportionation reaction of the cyclodextrin glucanotransferase from Klebsiella pneumoniae
Cyclodextrin glucanotransferases not only transform starch into cyclodextrins, but also catalyze the transfer of cyclodextrins to appropriate acceptors and the disproportionation of linear dextrins. These three reactions are manifestations of a single cata ...
1996Separation of functionalized dextrins by reversed-phase high-performance liquid chromatography
Functionalized linear dextrins were sepd. on a preparative scale by HPLC on RP-18 phases with water as the eluent. These dextrins were obtained by the transfer of a-cyclodextrin to glucosides by means of a cyclodextrin-glycosyltransferase of Klebsiella pne ...
1991The production of alpha-cyclodextrin by enzymatic degradation of starch
The maximum concentration of alpha-cyclodextrin for the enzymatic degradation of starch is limited to about 13.5 g X 1(-1). By addition of decanol, the equilibrium of the reaction system can be shifted towards an alpha-cyclodextrin yield of 50% even at hig ...
1984A photometric test for the cyclization activity of cyclodextrin-glycosyltransferases
A quick and reliable test was developed to measure the cyclization activity of cyclodextrin glycosyltransferases (CGT). Since the reproducibility depends mostly on the nature of the substrate, maltotriose was employed. The cyclodextrins formed are measured ...
1982