Concept
Endonucléase
Publications associées (39)
A ribonucleoprotein transfection strategy for CRISPR/Cas9-mediated gene editing and single cell cloning in rainbow trout cells
Background The advent of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology marked the beginning of a new era in the field of molecular biology, allowing the efficient and precise creation of targeted mutations in the ge ...
BMC2021Mechanistic insights into AMP-activated protein kinase-dependent gene expression
AMP-activated protein kinase (AMPK) is a fundamental enzyme that controls energy homeostasis, through orchestrating the cellular response to a reduction in energy availability. Under conditions of cellular energy stress AMPK senses the decrease in ATP leve ...
EPFL2019The evolution of multiple active site configurations in a designed enzyme
Ganna Gryn'ova, Dusan Petrovic
Developments in computational chemistry, bioinformatics, and laboratory evolution have facilitated the de novo design and catalytic optimization of enzymes. Besides creating useful catalysts, the generation and iterative improvement of designed enzymes can ...
NATURE PUBLISHING GROUP2018Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape
Georg Fantner, John McKinney, Haig Alexander Eskandarian
In most well-studied rod-shaped bacteria, peptidoglycan is primarily crosslinked by penicillin-binding proteins (PBPs). However, in mycobacteria, crosslinks formed by L,D-transpeptidases (LDTs) are highly abundant. To elucidate the role of these unusual cr ...
ELIFE SCIENCES PUBLICATIONS LTD2018Placeholder factors in ribosome biogenesis: please, pave my way
The synthesis of cytoplasmic eukaryotic ribosomes is an extraordinarily energy-demanding cellular activity that occurs progressively from the nucleolus to the cytoplasm. In the nucleolus, precursor rRNAs associate with a myriad of trans-acting factors and ...
Shared Science Publishers OG2017Role of microRNAs in tumor-associated macrophages
Tumor-associated macrophages (TAMs) infiltrate both mouse and human tumors, typically in high numbers, and display complex interactions with cancer cells, endothelial cells (ECs) and other immune cells. TAMs comprise molecularly distinct subsets. Two such ...
EPFL2016