Concept

Histone deacetylase inhibitor

Publications associées (94)

The Use of Valproic Acid in Transient Gene Expression with HEK-293E Cells

Transient gene expression (TGE) is a rapid method for the production of recombinant proteins. Recently, valproic acid (VPA), a well known histone deacetylase inhibitor, has been suggested for the use in cell cultures to enhance recombinant protein expressi ...

EPFL2013

Deletion of Sirt3 does not affect atherosclerosis but accelerates weight gain and impairs rapid metabolic adaptation in LDL receptor knockout mice: implications for cardiovascular risk factor development

Johan Auwerx, Julien Weber, Yuan Liu, Chad Brokopp

Sirt3 is a mitochondrial NAD(+)-dependent deacetylase that governs mitochondrial metabolism and reactive oxygen species homeostasis. Sirt3 deficiency has been reported to accelerate the development of the metabolic syndrome. However, the role of Sirt3 in a ...

Springer Verlag2013

An epigenetic blockade of cognitive functions in the neurodegenerating brain

Johannes Gräff

Cognitive decline is a debilitating feature of most neurodegenerative diseases of the central nervous system, including Alzheimer's disease. The causes leading to such impairment are only poorly understood and effective treatments are slow to emerge. Here ...

2012

Genome-Wide Histone Acetylation Is Altered in a Transgenic Mouse Model of Huntington's Disease

In Huntington's disease (HD; MIM ID # 143100), a fatal neurodegenerative disorder, transcriptional dysregulation is a key pathogenic feature. Histone modifications are altered in multiple cellular and animal models of HD suggesting a potential mechanism fo ...

Public Library Science2012

Targeting Sirtuin 1 to Improve Metabolism: All You Need Is NAD(+)?

Johan Auwerx, Carlos Canto Alvarez

Sirtuin 1 (SIRT1) is an evolutionarily conserved NAD(+)-dependent deacetylase that is at the pinnacle of metabolic control, all the way from yeast to humans. SIRT1 senses changes in intracellular NAD(+) levels, which reflect energy level, and uses this inf ...

2012

Structural Role of RKS Motifs in Chromatin Interactions: A Molecular Dynamics Study of HP1 Bound to a Variably Modified Histone Tail

Efthimios Kaxiras

The current understanding of epigenetic signaling assigns a central role to post-translational modifications that occur in the histone tails. In this context, it has been proposed that methylation of K9 and phosphorylation of S10 in the tail of histone H3 ...

2012

Sirtuins as regulators of metabolism and healthspan

Johan Auwerx, Richardus Houtkooper, Eija Riitta Pirinen

Since the beginning of the century, the mammalian sirtuin protein family (comprising SIRT1-SIRT7) has received much attention for its regulatory role, mainly in metabolism and ageing. Sirtuins act in different cellular compartments: they deacetylate histon ...

2012

The Kruppel-associated Box Repressor Domain Can Induce Reversible Heterochromatization of a Mouse Locus in Vivo

Didier Trono, Isabelle Barde, Sonia Verp, Sandra Eloise Kjeldsen, Anna Claire Groner

The study of chromatin and its regulators is key to understanding and manipulating transcription. We previously exploited the Krüppel-associated box (KRAB) transcriptional repressor domain, present in hundreds of vertebrate-specific zinc finger proteins, t ...

2012

Epigenetics of brain disorders

Johannes Gräff

This chapter discusses the epigenetics of brain disorders. Epigenetic mechanisms play a pivotal role in higher-order brain functions under physiological and pathological conditions. A deeper understanding of these mechanisms is of utmost importance for the ...

Elsevier2011

Large-Scale Transfection of Mammalian Cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Carine Meerschman

The large-scale transfection of mammalian cells allows moderate (milligram to gram) amounts of recombinant proteins (r-proteins) to be obtained for fundamental or clinical research. In this article, we describe a one-liter transfection using polyethyleneim ...

Humana Press2011

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