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A gene encoding a novel cdc2-related protein kinase has been identified in Plasmodium falciparum, using degenerate oligonucleotides designed to hybridise to regions that are conserved in members of the cdc2 gene family. This gene, called Pfcrk-1, is locate ...
To meet the need for studies of anaerobic microbial and animal cell cultures involving much lower heat effects as compared to aerobic microbial cultures, a bench scale calorimeter, Bio-RCl, has been improved for achieving a higher long-term sensitivity. Th ...
Batch cultures of a mouse/mouse hybridoma cell line secreting a dimeric IgA were performed in combination with a membrane process for the continuous removal of ammonia from the culture medium. The latter involves hydrophobic porous membranes with an assocd ...
The objective of this study was to clarify the relative roles of medial versus luminal factors in the induction of thickening of the arterial intima after balloon angioplasty injury. Platelet-derived growth factor (PDGF) and thrombin, both associated with ...
Brain-derived neurotrophic factor (BDNF) is a neurotrophic factor involved in neuronal development and synaptic plasticity. Although the physiological effects of BDNF have been examined in detail, target proteins which mediate its actions remain largely un ...
Many hormones, growth factors and neurotransmitters stimulate the production of inositol 1,4,5-trisphosphate evoking the release of calcium from intracellular stores. The depletion of these intracellular calcium stores subsequently activates a voltage-inde ...
We have identified by two-dimensional polyacrylamide gel electrophoresis a protein known as stathmin which is phosphorylated in a time- and concentration-dependent manner in response to brain-derived neurotrophic factor (BDNF) in primary cultures of cortic ...
Members of the nuclear receptor family are involved in an increasing number of processes in the cell. These receptors can directly regulate gene expression by binding to DNA. The molecular basis for transcriptional activation is so far not well understood. ...
In the present study, we screened for subtilisin-like proprotein convertases (SPCs) that potentially regulate the activation of known growth factors during embryonic development. We isolated a novel protease, SPC7, as well as several known SPCs. SPC7, like ...
Current evidence suggests that melanogenesis is controlled by epidermal paracrine modulators. We have analyzed the effects of transforming growth factor-beta1 (TGF-beta1) on the basal melanogenic activities of B16/F10 mouse melanoma cells. TGF-beta1 treatm ...