Concept
ADN polymérase bêta
Publications associées (20)
Telomere protection against oxidative stress
Telomeres are nucleoprotein structures at the ends of linear chromosomes, being essential for the maintenance of genomic integrity. Telomeres have a unique structure which distinguishes chromosome termini from DNA damage sites. Shelterin complexes are the ...
EPFL2023Requirements for DNA bubble structure for efficient cleavage by helix-two-turn-helix DNA glycosylases
Oxidative DNA lesions, constantly generated by both endogenous and environmentally induced reactive oxygen species, are removed via the base excision repair pathway. In bacteria, Fpg and Nei DNA glycosylases, belonging to the helix-two-turn-helix (H2TH) st ...
2020A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
Double-strand breaks (DSBs) are the most toxic type of DNA lesions. Cells repair these lesions using either end protection- or end resection-coupled mechanisms. To study DSB repair choice, we present the Color Assay Tracing-Repair (CAT-R) to simultaneously ...
2020Long noncoding RNA SNHG12 integrates a DNA-PK-mediated DNA damage response and vascular senescence
Johan Auwerx, Norman Lionel Moullan, Lei Chen
Long noncoding RNAs (lncRNAs) are emerging regulators of biological processes in the vessel wall; however, their role in atherosclerosis remains poorly defined. We used RNA sequencing to profile lncRNAs derived specifically from the aortic intima of Ldlr(- ...
AMER ASSOC ADVANCEMENT SCIENCE2020Preexisting variation in DNA damage response predicts the fate of single mycobacteria under stress
John McKinney, Neeraj Dhar, Giulia Manina, Lalit Kumar Singh
Clonal microbial populations are inherently heterogeneous, and this diversification is often considered as an adaptation strategy. In clinical infections, phenotypic diversity is found to be associated with drug tolerance, which in turn could evolve into g ...
2019Cooperative motion of a key positively charged residue and metal ions for DNA replication catalyzed by human DNA Polymerase-eta
Trans-lesion synthesis polymerases, like DNA Polymerase-eta (Pol-eta), are essential for cell survival. Pol-eta bypasses ultraviolet-induced DNA damages via a two-metal-ion mechanism that assures DNA strand elongation, with formation of the leaving group p ...
Oxford University Press (OUP)2016Protein ruthenation and DNA alkylation: chlorambucil-functionalized RAPTA complexes and their anticancer activity
Paul Joseph Dyson, Christian Hartinger, Alexey Nazarov, Olivier Pierre Zava
Chemotherapeutics for the treatment of tumorigenic conditions that feature novel modes of action are highly sought after to overcome the limitations of current chemotherapies. Herein, we report the conjugation of the alkylating agent chlorambucil to the RA ...
2015From polymers to gene regulation
DNA molecule is the fundamental component of every living organism, since it encodes the hereditary information. Although the genetic code of almost 200 species has been sequenced, the direct connection between gene sequence, DNA structure and biological f ...
EPFL2015Combining RNAi and in vivo confocal microscopy analysis of the photoconvertible fluorescent protein Dendra2 to study a DNA repair protein
Clinical approaches for tumor treatment often rely on combination therapy where a DNA damaging agent is used in combination with a DNA repair protein inhibitor. For this reason, great efforts have been made during the last decade to identify inhibitors of ...
Biotechniques Office2013Insights into Intrastrand Cross-Link Lesions of DNA from QM/MM Molecular Dynamics Simulations
Ursula Röthlisberger, Chandan Patel
DNA damages induced by oxidative intrastrand cross-links have been the subject of intense research during the past decade. Yet, the currently available experimental protocols used to isolate such lesions only allow to get structural information about linke ...
2012