Concept

In-gel digestion

Publications associées (36)

Empirical multi-dimensional space for scoring peptide spectrum matches in shotgun proteomics

Data-dependent tandem mass spectrometry (MS/MS) is one of the main techniques for protein identification in shotgun proteomics. In a typical LC MS/MS workflow, peptide product ion mass spectra (MS/MS spectra) are compared with those derived theoretically f ...

Amer Chemical Soc2014

Light diffusion in GFRP laminates for building construction

Thomas Keller, Andreas Schueler, André Gabriel Kostro, Julia De Castro San Roman Fest, Carlos Pascual Agullo

The light diffusion of hand lay-up glass fiber-reinforced polymer (GFRP) laminates was investigated with a spectrophotometric set-up using an integrating sphere. Reinforcement weights of the laminates ranged from 410 to 3280 g/m2 and fiber volume fractions ...

2014

From food to physiology characterization and in vitro digestion of milk products and investigation of postprandial metabolism and inflammation after a high-fat meal intake in a human nutrition intervention study

Flurina Schwander

According to the World Health Organization worldwide obesity has more than doubled since 1980. The World health statistics 2012 report shows that one in six adults is obese, one in ten diabetic and one in three has a raised blood pressure. Overweight and o ...

EPFL2013

Encapsulation of Huh-7 cells within alginate-poly(ethylene glycol) hybrid microspheres

Jutta Christine Wandrey, Redouan Mahou

Novel calcium alginate poly(ethylene glycol) hybrid microspheres (Ca-alg-PEG) were developed and evaluated as potentially suitable materials for cell microencapsulation. Grafting 5-13% of the backbone units of sodium alginate (Na-alg) with alpha-amine-omeg ...

Springer US2012

Versatile Route to Synthesize Heterobifunctional Poly(ethylene glycol) of Variable Functionality for Subsequent Pegylation

Jutta Christine Wandrey, Redouan Mahou

Pegylation using heterotelechelic poly(ethylene glycol) (PEG) offers many possibilities to create high-performance molecules and materials. A versatile route is proposed to synthesize heterobifunctional PEG containing diverse combinations of azide, amine, ...

Mdpi Ag2012

Macroporous Materials as Novel Catalysts for Efficient and Controllable Proteolysis

Liang Qiao, Jingjing Wan, Jiawei Tang

A novel nanopore based digestion strategy has been developed by directly adding a macroporous material as catalyst to the conventional in-solution reaction system. Without increasing the enzyme or protein concentrations, this simple digestion approach exhi ...

American Chemical Society2009

Tailoring hydrogel degradation and drug release via neighboring amino acid-controlled ester hydrolysis

Matthias Lütolf, Jeffrey Alan Hubbell, Yun Suk Jo, Jay Gantz

We present a versatile scheme to rationally modulate the hydrolysis rate of ester bonds in hydrophilic polymer networks via adjacent charged amino acids. As soluble model systems, two cysteine-bearing oligopeptides containing either positively charged argi ...

2009

A Phospho-Directed Macroporous Alumina-Silica Nanoreactor with Multi-Functions

Hubert Girault, Fang Liu, Jingjing Wan

A phospho-directed nanoreactor with multiple functions is reported. Alumina-functionalized macroporous ordered silica foams (Al-MOSF) have been developed with large pore size, high pore volume (1.6 cm(3)/g), and a surface area of 186 m(2)/g rich in coordin ...

American Chemical Society2009

Of gels, offgel and off with the gel

Hoang-Trang Lam

In the context of prefractionation methods for proteomics, this work deals mainly with the development of electrophoretic tools for isoelectric focusing of peptides and proteins for the analysis of biological mixtures. In the light of existing devices for ...

EPFL2008

Microfluidic enzymatic reactors for proteome research

Hubert Girault

The field of proteomics has emerged as a valuable analytical tool for elucidating cellular and biological systems at the molecular level. As there is an ever-growing demand for new highly automated, high-throughput, and sensitive analytical tools, a key ch ...

2008

Graph Chatbot

Chattez avec Graph Search

Posez n’importe quelle question sur les cours, conférences, exercices, recherches, actualités, etc. de l’EPFL ou essayez les exemples de questions ci-dessous.

AVERTISSEMENT : Le chatbot Graph n'est pas programmé pour fournir des réponses explicites ou catégoriques à vos questions. Il transforme plutôt vos questions en demandes API qui sont distribuées aux différents services informatiques officiellement administrés par l'EPFL. Son but est uniquement de collecter et de recommander des références pertinentes à des contenus que vous pouvez explorer pour vous aider à répondre à vos questions.

Connectez-vous pour utiliser Chat avec Graph Search