Concept

Protein production

Publications associées (160)

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Beta-glucosidase (BGL) is a rate-limiting enzyme for cellulose hydrolysis as it acts in the final step of lignocellulosic biomass conversion to convert cellobiose into glucose, the final end product. Most of the fungal strains used for cellulase production ...

Elsevier2017

Cytotoxic Half-Sandwich Rh(III) and Ir(III) beta-Diketonates

Paul Joseph Dyson, Rosario Scopelliti, Tina Riedel, Agnese Petrini

A series of half-sandwich pentamethylcyclopentadienyl rhodium(III) and iridium(III) complexes [CpM(DBM/ HDB/AVB)Cl] and [CpM(DBM/HDB/AVB)(PTA)]-[SO3CF3], where Cp* = pentamethylcyclopentadienyl, the proligands DBMH = dibenzoylmethane, HDBH = o-hydroxydi- ...

American Chemical Society2017

Multigene expression in stable CHO cell pools generated with the piggyBac transposon system

Florian Maria Wurm, David Hacker, Sowmya Balasubramanian

Heterogenous populations of recombinant cells (cell pools) stably expressing 1-4 transgenes were generated from Chinese hamster overy (CHO) cells with the piggyBac (PB) transposon system. The cell pools produced different combinations of three model protei ...

American Chemical Society2016

We highlight recent developments for the production of recombinant proteins from suspension-adapted mammalian cell lines. We discuss the generation of stable cell lines using transposons and lentivirus vectors (non-targeted transgene integration) and site- ...

Elsevier2016

Mens Sana in Corpore Sano

Christophe Alhanko

Lieu dédié au corps et à l'esprit, le programme est déterminé par des objectifs multiples propices à l'exercice physique et à la flânerie. Bains, piscines et équipements sportifs se regroupent pour former une nouvelle condition, sorte d'exutoire au sein d' ...

2016

Comparison of Three Transposons for the Generation of Highly Productive Recombinant CHO Cell Pools and Cell Lines

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Sowmya Balasubramanian

Several naturally occurring vertebrate transposable elements have been genetically modified to enable the transposition of recombinant genes in mammalian cells. We compared three transposonspiggyBac, Tol2, and Sleeping Beautyfor their ability to generate c ...

Wiley-Blackwell2016

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Short alpha-helical peptides stabilized by linkages between constituent amino acids offer an attractive format for ligand development. In recent years, a range of excellent ligands based on stabilized alpha-helices were generated by rational design using a ...

2016

A High-Throughput Microfluidic Platform for Mammalian Cell Transfection and Culturing

Sebastian Maerkl, Kristina Pan Woodruff

Mammalian synthetic biology could be augmented through the development of high-throughput microfluidic systems that integrate cellular transfection, culturing, and imaging. We created a microfluidic chip that cultures cells and implements 280 independent t ...

Nature Publishing Group2016

Growth factors are important morphogenetic proteins that instruct cell behavior and guide tissue repair and renewal. Although their therapeutic potential holds great promise in regenerative medicine applications, translation of growth factors into clinical ...

Elsevier Sci Ltd2016

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Orbitally shaken bioreactors (OSRs) support the suspension cultivation of animal cells at volumetric scales up to 200 L and are a potential alternative to stirred-tank bioreactors (STRs) due to their rapid and homogeneous mixing and high oxygen transfer ra ...

American Chemical Society2016