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We have used monospecific antibodies against three ribonuclease H enzymes of Saccharomyces cerevisiae to investigate their intracellular localization. Fractionation experiments, as well as immunocytochemical staining, revealed a predominantly cytoplasmic l ...
Yeasts degrade glucose through different metabolic pathways, where the choice of the pathway is dependent on the nature of the limitation in the various substrates. When oxygen is limiting in addn. to glucose, yeasts often grow according to a mixt. of oxid ...
A new type of miniature bioreactor for continuous culture of yeast cells in space laboratories has been developed. Silicon microtechnology has permitted the integration of numerous functions and systems in a volume of 87 x 63 x 63 mm3 and a weight of 610 g ...
Poly(A) polymerase was purified 22,000-fold to homogeneity from a whole cell extract of Saccharomyces cerevisiae with a yield of 22%. The enzyme is a monomeric polypeptide with a denatured molecular weight of 63,000. Incorporation of labeled ATP into acid- ...
A prior genetic study indicated that activity of Sir silencing proteins at a hypothetical AGE locus is essential for long life span. In this model, the SIR4-42 mutation would direct the Sir protein complex to the AGE locus, giving rise to a long life span. ...
Aerobic chemostat cultures of Saccharomyces cerevisiae were performed under carbon-, nitrogen-, and dual carbon- and nitrogen-limiting conditions. The glucose concn. was kept const., whereas the ammonium concn. was varied among different expts. and differe ...
Chemostat cultures of an aerobic fermenting yeast (Saccharomyces cerevisiae) and an aerobic respiring yeast (Kluyveromyces fragilis) were grown under dual C + N limitation in an isothermal reaction calorimeter. The dual limitations resulted in uncoupled ox ...
The growth physiol. of S. cerevisiae strains H1022 and Whi2+ was studied in aerobic batch and continuous (chemostat) cultures. Results from the measurement of biomass and medium components (off-line) together with O2, CO2, and heat measurements (online) we ...
