Concept
Transfert de protéines
Publications associées (95)
Differential Cytotoxicity, Cellular Uptake, Apoptosis and Inhibition of BRCA1 Expression of BRCA1-Defective and Sporadic Breast Cancer Cells Induced by an Anticancer Ruthenium(II)-Arene Compound, RAPTA-EA1
Background: The RAPTA-EA1 complex [ruthenium(II)-arene 1,3,5-triaza-7-phosphaadamantane (pta) complex with an arene-tethered ethacrynic acid ligand] has been reported to overcome drug resistance that developed due to the current use of platinum-based treat ...
Bentham Science Publ Ltd2017Microfluidic staining technology and automated image processing for fast and accurate tissue-based diagnostics
The development of microfluidics has led to significant improvements in many areas of in vitro diagnostics. This thesis proposes the use of microfluidic device technology in the field of anatomic pathology, leading to faster and more accurate tissue-based ...
EPFL2016Optimisation of aqueous synthesis of iron oxide nanoparticles for biomedical applications
Heinrich Hofmann, Marijana Mionic Ebersold, Débora Bonvin
Iron oxide nanoparticles (IONPs) were prepared via aqueous synthesis which combines alkaline co-precipitation (CP) of ferric and ferrous precursors with mild hydrothermal (HT) treatment without cupping agents (CA). In this novel synthesis route, CP + HT, w ...
Springer Verlag2016Soft nanofluidics governing minority ion exclusion in charged hydrogels
Fabien Patrick Wildhaber, Songmei Wu, Thomas Michael Braschler, Sidi Ahmed Bencherif
We investigate ionic partition of negatively charged molecular probes into also negatively charged, covalently crosslinked alginate hydrogels. The aim is to delimit the domain of validity of the major nanoelectrostatic models, and in particular to assess t ...
Royal Society of Chemistry2015Porous silica enhanced proteolysis during Off-Gel separation for efficient protein identification
Hubert Girault, Liang Qiao, Mohammad Reza Pourhaghighi
An advanced approach is developed in this work for simultaneous on-line separation and digestion of proteins by combining the Off-Gel isoelectric focusing (IEF) and enzymatic nanoreactor enhanced proteolysis. The nanoreactor was prepared by preloading tryp ...
Elsevier2015SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy
Kai Johnsson, Luc Reymond, Alberto Schena, Grazvydas Lukinavicius
Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utilit ...
2015The influence of microchemistry on the recrystallization texture of cold-rolled Al-Mn-Fe-Si alloys
The recrystallization textures of a cold-rolled Al-Mn-Fe-Si model alloy with three different microchemistry states after non-isothermal annealing were studied. The microstructure and texture evolution have been characterized by EBSD. It is clearly demonstr ...
Institute of Physics2015Component-resolved diagnostic of cow’s milk allergy by immunoaffinity capillary electrophoresis – matrix assisted laser desorption/ionization mass spectrometry
Hubert Girault, Natalia Gasilova
Component-resolved diagnostic (CRD) of cow’s milk allergy has been performed using immunoaffinity capillary electrophoresis (IACE) coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). First, total IgE quantification in the ...
Amer Chemical Soc2014The Phosphate Specific Transport (Pst) System in Fast- and Slow-Growing Mycobacteria
Although mycobacteria are known to accumulate polyphosphate (PolyP), a linear polymer of orthophosphate, when subjected to stresses, the exact roles of this molecule are not yet clearly understood. We found that deletion of the pstA gene encoding for a tra ...
EPFL2013Functional genotyping of Sulfurospirillum spp. in mixed cultures allowed the identification of a new tetrachloroethene reductive dehalogenase
Christof Holliger, Julien Maillard, Géraldine Florence Buttet
Background Several enrichment cultures were investigated for their bioremediation potential of tetrachloroethene (PCE). The underlying process (organohalide respiration, OHR) is an anaerobic bacterial respiration during which the chlorinated compounds are ...
2013