Concept
Transfert de protéines
Publications associées (95)
Urtica dioica Leaf Infusion Enhances the Sensitivity of Triple-Negative Breast Cancer Cells to Cisplatin Treatment
Urtica dioica (UD) has been widely used in traditional medicine due to its therapeutic benefits, including its anticancer effects. Natural compounds have a promising potential when used in combination with chemotherapeutic drugs. The present study explores ...
MDPI2023Living Photovoltaics based on Recombinant Expression of MtrA Decaheme in Photosynthetic Bacteria
Ardemis Anoush Boghossian, Melania Reggente, Mohammed Mouhib, Alessandra Antonucci, Nils Schürgers, Sara Politi
At the center of microbial bioelectricity applications lies the critical need to express foreign heme proteins that are capable of redirecting the electron flux of the cell’s metabolism. This study presents bioengineered Synechocystis sp. PCC 6803 cells ca ...
2023The Antioxidant and Proapoptotic Effects of Sternbergia clusiana Bulb Ethanolic Extract on Triple-Negative and Estrogen-Dependent Breast Cancer Cells In Vitro
Natalia Gasilova, Laure Menin, Maria Younes
Background: Sternbergia clusiana belongs to the Amaryllidaceae family and is recognized for the valuable biological activity of its major bioactive compounds. The aim of the current is to evaluate the anticancer effects of the ethanolic bulb extract of Ste ...
MDPI2023Acoustofluidic large-scale mixing for enhanced microfluidic immunostaining for tissue diagnostics
Martinus Gijs, Diego Gabriel Dupouy, Muaz Salama Abdelmonem Draz
The usage of microfluidics for automated and fast immunoassays has gained a lot of interest in the last decades. This integration comes with certain challenges, like the reconciliation of laminar flow patterns of micro-scale systems with diffusion-limited ...
ROYAL SOC CHEMISTRY2023Dopamine Transporter, PhosphoSerine129 alpha-Synuclein and alpha-Synuclein Levels in Aged LRRK2 G2019S Knock-In and Knock-Out Mice
Salvatore Novello, Chiara Domenicale
The G2019S mutation in leucine rich-repeat kinase 2 (LRRK2) is a major cause of familial Parkinson's disease. We previously reported that G2019S knock-in mice manifest dopamine transporter dysfunction and phosphoSerine129 alpha-synuclein (pSer129 alpha-syn ...
MDPI2022Controlling the immobilization process of an optically enhanced protein microarray for highly reproducible immunoassay
By virtue of its high throughput multiplex detection capability, superior read-out sensitivity, and tiny analyte consumption, an optically enhanced protein microarray assay has been developed as a promising diagnostic tool for various applications, ranging ...
ROYAL SOC CHEMISTRY2021A simple micropreparative gel electrophoresis technique for purification of proteins, nucleic acids, and bioconjugates
Ardemis Anoush Boghossian, Sayyed Hashem Sajjadi, Shang-Jung Wu, Vitalijs Zubkovs
The biochemical and biomedical fields hinge on the ability to effectively separate and purify biological macromolecules. Though this need is largely addressed with a variety of chromatographic and electrophoretic purification techniques, such techniques ar ...
2021How specific are the conformation-specific α-synuclein antibodies? Characterization and validation of 16 α-synuclein conformation-specific antibodies using well-characterized preparations of α-synuclein monomers, fibrils and oligomers with distinct structures and morphology
Hilal Lashuel, Somanath Jagannath
Increasing evidence suggests that alpha-synuclein (α-syn) oligomers are obligate intermediates in the pathway involved in α-syn fibrillization and Lewy body (LB) formation, and may also accumulate within LBs in Parkinson's disease (PD) and other synucleino ...
2020MicroRNA-204-5p modulates mitochondrial biogenesis in C2C12 myotubes and associates with oxidative capacity in humans
Using an unbiased high-throughput microRNA (miRNA)-silencing screen combined with functional readouts for mitochondrial oxidative capacity in C2C12 myocytes, we previously identified 19 miRNAs as putative regulators of skeletal muscle mitochondrial metabol ...
2020Establishing a Ternary System for Optical Monitoring of DNA-Protein Interactions with Single-Walled Carbon Nanotubes
DNA-protein interactions lie at the crux of life's essential processes. As such, various technologies have been developed to characterize these interactions. The distinct advantages of these technologies can be leveraged to study different facets of these ...
EPFL2020