Publications associées à Pharming (biologie)

OnePot PURE Cell-Free System

Sebastian Maerkl, Laura Sophie Grasemann, Barbora Lavickova

The defined PURE (protein synthesis using recombinant elements) transcription-translation system provides an appealing chassis for cell-free synthetic biology. Unfortunately, commercially available systems are costly, and their tunability is limited. In co ...

2021

Legacy effect of green manure crops fertilized with calcium phosphite on maize production and soil properties

Alexandre Buttler, Luca Bragazza, Thomas Guillaume, Mathieu Raymond Santonja, Mario Fontana

Recycling phosphorus (P) is crucial to meet future P demand for crop production. We investigated the possibility to use calcium phosphite (Ca-Phi) waste, an industrial by-product, as P fertilizer following the oxidation of phosphite (Phi) to phosphate (Pi) ...

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD2021

The Use of Valproic Acid in Transient Gene Expression with HEK-293E Cells

Divor Kiseljak

Transient gene expression (TGE) is a rapid method for the production of recombinant proteins. Recently, valproic acid (VPA), a well known histone deacetylase inhibitor, has been suggested for the use in cell cultures to enhance recombinant protein expressi ...

EPFL2013

Transient Gene Expression in HEK-293E Cells for Recombinant Protein Production : Process Characterization towards GMP Approval

Sophie Nallet

The growing demand of biopharmaceutical products is boosting the market for therapeutic recombinant proteins (r-proteins). More than half of the 140 r-proteins that have gained approval for human therapeutic use are manufactured in mammalian cells that mak ...

EPFL2010

Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Xiaowei Zhang, Isabel Fernandez Garcia

The effect of hyperosmolarity on transient recombinant protein production in Chinese hamster ovary (CHO) cells was investigated. Addition of 90 mM NaCl to the production medium ProCHO5 increased the volumetric yield of recombinant antibody up to 4-fold rel ...

Springer Netherlands2010

Transient recombinant protein expression in mammalian cells : the role of mRNA level and stability

Sarah Wulhfard

Transient gene expression (TGE) is a rapid method for generating recombinant proteins in mammalian cells, but the volumetric productivities for secreted proteins in transiently transfected CHO DG44 cells are typically more than an order of magnitude lower ...

EPFL2009

Increased tolerance of sunflower mutant seedlings to Cd and Zn in hydroponic culture

Jean-Paul Schwitzguebel, Erika Nehnevajova

Cadmium and zinc show similarities in their chemistry, geochemistry and environmental properties. Whereas Cd is a phytotoxic non-essential element, Zn is an essential trace element for plant growth and development. Zinc plays a fundamental role in several ...

2009

Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells

Florian Maria Wurm, David Hacker, Stéphanie Tissot, Sarah Wulhfard, Sophie Bouchet, Jean Cevey

Large-scale transient gene expression (TGE) in mammalian cells is a rapid method to generate recombinant proteins, but the volumetric productivity for secreted proteins is still more than an order of magnitude lower than the yields typically achieved with ...

2008

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

Florian Maria Wurm, David Hacker, Martin Jordan, Madiha Derouazi, Natalie Muller, Sarah Wulhfard

Cell expansion, gene transfer and protein production were all executed with a single serum-free, animal protein-free commercial medium designed for suspension-adapted Chinese hamster ovary cells (CHO DG44). This is a most important process to consider for ...

Kluwer Academic Publishers, Springer2007