Concept
Prométaphase
Publications associées (13)
Identification and characterization of proteins that protect human telomeres from fragility
Difficulties to replicate telomeres - the ends of our chromosomes - can cause telomere shortening andgenome instability. These difficulties are due to the repetitive DNA sequence and distinct structures at telomeresthat challenge the semi-conservative DNA ...
EPFL2022Challenging endings: How telomeres prevent fragility
It has become apparent that difficulties to replicate telomeres concern not only the very ends of eukaryotic chromosomes. The challenges already start when the replication fork enters the telomeric repeats. The obstacles encountered consist mainly of nonca ...
WILEY2021Revealing chromatin organization in metaphase chromosomes
The local structural organization of chromatin in mitotic chromosomes is not well understood. A new cryo-electron tomography study from the Daban laboratory (Chicano et al, ) reveals that mitotic chromatin isolated from human cells can assume a plate-like ...
WILEY2019Infrared nanospectroscopic mapping of a single metaphase chromosome
Giovanni Dietler, Andrzej Kulik, Francesco Simone Ruggeri, Ewelina Wioletta Lipiec
The integrity of the chromatin structure is essential to every process occurring within eukaryotic nuclei. However, there are no reliable tools to decipher the molecular composition of metaphase chromosomes. Here, we have applied infrared nanospectroscopy ...
Oxford University Press (OUP)2019Zika virus causes supernumerary foci with centriolar proteins and impaired spindle positioning
Pierre Gönczy, Coralie Busso, Benita Wolf
Zika virus (ZIKV) causes congenital microcephaly. Although ZIKV can impair cell cycle progression and provoke apoptosis, which probably contributes to disease aetiology through depletion of neural progenitor cells, additional cellular mechanisms may be imp ...
Royal Society, The2017Mechanisms of centrosome separation in C. elegans
Centrosomes are the major microtubule organizing centers of animal cells. The two centrosomes present at the onset of mitosis must separate in a timely fashion along the nuclear envelope to ensure proper bipolar spindle assembly and thus genome stability. ...
EPFL2016Analysis of S. pombe SIN protein association to the SPB reveals two genetically separable states of the SIN
Michaël Unser, Daniel Sage, Jacques Rougemont, Viesturs Simanis, Andrea Krapp, Daniel Andreas Schmitter, Anastasia Chasapi, Ioannis Xenarios
The Schizosaccharomyces pombe septation initiation network (SIN) regulates cytokinesis, and asymmetric association of SIN proteins with the mitotic spindle pole bodies (SPBs) is important for its regulation. Here, we have used semi-automated image analysis ...
Company Of Biologists Ltd2015A 2D/3D image analysis system to track fluorescently labeled structures in rod-shaped cells: application to measure spindle pole asymmetry during mitosis
Michaël Unser, Daniel Sage, Viesturs Simanis, Daniel Andreas Schmitter, Anastasia Chasapi, Ioannis Xenarios
Background: The yeast Schizosaccharomyces pombe is frequently used as a model for studying the cell cycle. The cells are rod-shaped and divide by medial fission. The process of cell division, or cytokinesis, is controlled by a network of signaling proteins ...
Biomed Central Ltd2013Selective Chemical Crosslinking Reveals a Cep57-Cep63-Cep152 Centrosomal Complex
Kai Johnsson, Pierre Gönczy, Luc Reymond, Grazvydas Lukinavicius, Keitaro Umezawa
The centrosome functions as the main microtubule-organizing center of animal cells and is crucial for several fundamental cellular processes [1]. Abnormalities in centrosome number and composition correlate with tumor progression [2, 3] and other diseases ...
Elsevier2013Phosphorylation state defines discrete roles for monopolin in chromosome attachment and spindle elongation
BACKGROUND: It is unknown how oscillations in Cdk1 activity drive the dramatic changes in chromosome and spindle dynamics that occur at the metaphase/anaphase transition. RESULTS: We show that the Schizosaccharomyces pombe monopolin complex has distinct fu ...
2009