Concept

Lactase

Publications associées (30)

The FIP1 gene encodes a component of a yeast pre-mRNA polyadenylation factor that directly interacts with poly(A) polymerase

We have identified an essential gene, called FIP1, encoding a 327 amino acid protein interacting with yeast poly(A) polymerase (PAP1) in the two-hybrid assay. Recombinant FIP1 protein forms a 1:1 complex with PAP1 in vitro. At 37 degrees C, a thermosensiti ...

1995

Mouse interleukin-2 receptor alpha gene expression. Interleukin-1 and interleukin-2 control transcription via distinct cis-acting elements

Philipp Bucher

We have shown that interleukin-1 (IL-1) and IL-2 control IL-2 receptor alpha (IL-2R alpha) gene transcription in CD4-CD8- murine T lymphocyte precursors. Here we map the cis-acting elements that mediate interleukin responsiveness of the mouse IL-2R alpha g ...

1995

Glycerol production by yeast fermentation of whey permeate

Urs von Stockar, Ian William Marison

Yeast strains were examd. for their ability to produce glycerol, and Kluyveromyces marxianus NRRLY-665 was chosen for further optimization due to its comparatively high growth rate (0.15/h) and glycerol yield (9.5% by wt. on lactose) at 37 Deg and pH 7. Op ...

1994

The influence of the ion content of whey on the pH-activity profile of the b-galactosidase from Aspergillus oryzae

Albert Renken

The pH-dependence of the reaction kinetics of lactase (b-galactosidase) [9031-11-2] from A. oryzae in different reaction media is presented in terms of a 2-parameter model. The lactase from A. oryzae seems to have replaced the A. niger lactase on the marke ...

1985

Deactivation kinetics and temperature optimization of discontinuous enzymic processes

Albert Renken

The influence of temp. on the catalytic activity and deactivation of sol. enzymes which are com. used in batch processes is discussed. Since high temp. not only accelerates the desired enzymic reaction but also the undesired deactivation of the enzyme, it ...

1985

Alcohol production from whey permeate by yeast and by a thermophile strain

Urs von Stockar

The potential of EtOH [64-17-5] fermn. of whey permeate as a means of whey disposal and utilization in Switzerland was explored. The effluent lactose content of unconcd. permeate can be reduced to 2% of the original value by continuous fermn. with Kluyvero ...

1985

Metabolic, physiological and kinetic aspects of the alcoholic fermentation of whey permeate by Kluyveromyces fragilis NRRL 665 and Kluyveromyces lactis NCYC 571

Urs von Stockar

Optimum growth conditions for the fermn. of nonconcd. whey permeate by K. fragilis NRRL 665 were defined. Use of 3.75 g yeast ext./L, a growth temp. of 38 Deg and a pH of 4.0 allowed a max. productivity of 5.23 g EtOH [64-17-5]/L-h in continuous culture wi ...

1985

An investigation of ethanol inhibition and other limitations occurring during the fermentation of concentrated whey permeate by Kluyveromyces fragilis

Urs von Stockar

Based on the well-known fact that K. fragilis strains show suboptimal performance when grown in concd. whey permeate, previously optimized medium was investigated for possible limitations appearing at high concns. Shaken flask cultures showed that no addnl ...

1985

Development of an enzyme fluidized bed reactor equipped with static mixers: application to lactose hydrolysis in whey

Albert Renken

Reactor operation with immobilized enzymes in fixed bed arrangement is often impaired due to the presence of finely divided solid matter, adsorbing substances or gas. The fluidized bed reactor would be applied in such cases owing to a limited pressure drop ...

1984

The kinetics of lactose hydrolysis for the b-galactosidase from Aspergillus niger

Albert Renken

The kinetics of glucose liberation from lactose by b-galactosidase from A. niger was studied in a wide range of the main variables. The anal. shows that the kinetic models proposed so far are not adequate. The main finding is that the reaction rate is not ...

1982

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