Concept

Levure de boulanger

Publications associées (79)

Activation of the unfolded protein response pathway causes ceramide accumulation in yeast and INS-1E insulinoma cells

Sphingolipids are not only important components of membranes but also have functions in protein trafficking and intracellular signaling. The LCB1 gene encodes a subunit of the serine palmitoyltransferase, which is responsible for the first step of sphingol ...

2012

Rapid Synthesis of Defined Eukaryotic Promoter Libraries

Sebastian Maerkl, Arun Stephen Rajkumar

Current gene synthesis methods allow the generation of long segments of dsDNA. We show that these techniques can be used to create synthetic regulatory elements and describe a method for the creation of completely defined, synthetic variants of the PHOS pr ...

Amer Chemical Soc2012

Telomeric Repeat Containing RNAs and the Response to Telomere Deprotection in Saccharomyces Cerevisiae

Andrea Bernardo Panza

Telomeres are the DNA/RNA/protein structures at the end of linear eukaryotic chromosomes, which protect them against the DNA damage repair machinery, preventing chromosome end-to-end fusions and aberrant recombination. Moreover, telomeres compensate for th ...

EPFL2012

Bacterial Selection during the Formation of Early-Stage Aerobic Granules in Wastewater Treatment Systems Operated Under Wash-Out Dynamics

Christof Holliger, Julien Maillard, Pierre Rossi, David Gregory Weissbrodt, Samuel Lochmatter, Sirous Ebrahimi

Aerobic granular sludge is attractive for high-rate biological wastewater treatment. Biomass wash-out conditions stimulate the formation of aerobic granules. Deteriorated performances in biomass settling and nutrient removal during start-up have however of ...

2012

Production of a thermostable fumarase in Saccharomyces cerevisiae for the bioconversion of fumarate to L-malate

Coralie Signorell

The gene encoding fumarase (fum) from Thermus thermophilus was expressed in yeast Saccharomyces cerevisiae. The recombinant cells were heated at 70°C to inactivate indigenous enzymes and used for the bioconversion of fumaric acid to L-malic acid. By heatin ...

2011

Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity

Philippe Renaud, Thomas Michael Braschler, Nicolas Demierre, Ana Valero

Cell cycle synchronization is an important tool for the study of the cell division stages and signalling. It provides homogeneous cell cultures that are of importance to develop and improve processes such as protein synthesis and drug screening. The main a ...

Royal Society of Chemistry2011

Multiple-frequency Impedance Measurements in Continuous Flow for the Evaluation of Electrical Lysis of Yeast Cells

Philippe Renaud, Niccolo Piacentini, Willyan Hasenkamp Carreira

his paper reports on an electrical lysis device with integrated cell counting and evaluation of the lysis efficiency by multiplefrequency impedance measurements. Conventional techniques use chemical reagents to lyse cells, causing unwanted interference wit ...

Elsevier2010

A miniaturized continuous dielectrophoretic cell sorter and its applications

Philippe Renaud, Thomas Michael Braschler, Nicolas Demierre, Ana Valero

There is great interest in highly sensitive separation methods capable of quickly isolating a particular cell type within a single manipulation step prior to their analysis. We present a cell sorting device based on the opposition of dielectrophoretic forc ...

American Institute of Physics2010

WebPrInSeS: automated full-length clone sequence identification and verification using high-throughput sequencing data

Bart Deplancke, Andreas Massouras, Frederik Decouttere

High-throughput sequencing (HTS) is revolutionizing our ability to obtain cheap, fast and reliable sequence information. Many experimental approaches are expected to benefit from the incorporation of such sequencing features in their pipeline. Consequently ...

Oxford University Press2010

Label-free Sorting and Counting of Yeast Cells for Viability Studies

Philippe Renaud, Niccolo Piacentini, Raphaël Tornay, Nina Buffi

This paper reports the new combination of cell sorting and counting capabilities on a single device. Most state-of-the-art devices combining these technologies use optical techniques requiring complicate experimental setups and labeled samples. The use of ...

Elsevier2009

Graph Chatbot

Chattez avec Graph Search

Posez n’importe quelle question sur les cours, conférences, exercices, recherches, actualités, etc. de l’EPFL ou essayez les exemples de questions ci-dessous.

AVERTISSEMENT : Le chatbot Graph n'est pas programmé pour fournir des réponses explicites ou catégoriques à vos questions. Il transforme plutôt vos questions en demandes API qui sont distribuées aux différents services informatiques officiellement administrés par l'EPFL. Son but est uniquement de collecter et de recommander des références pertinentes à des contenus que vous pouvez explorer pour vous aider à répondre à vos questions.

Connectez-vous pour utiliser Chat avec Graph Search