Concept
Mutagénèse
Publications associées (33)
Use of site-directed mutagenesis to probe structure-function relationships of alpha-toxin from Clostridium perfringens.
The NH2-terminal domain of the alpha-toxin of Clostridium perfringens is highly homologous to the complete phospholipase C from Bacillus cereus (PC-PLC), for which a high-resolution crystal structure is available. This structural information was used as th ...
1996A statistical model to estimate variance in long term-low dose mutation assays: testing of the model in a human lymphoblastoid mutation assay
Pramod Rastogi, Stephan Morgenthaler
Long term-low dose mutation assays offer a means to study the genetic effects of environmental mutagens at concentrations relevant to human exposure. These assays involve continuous induction of mutants, serial dilution of cultures and sampling to determin ...
1989beta-Galactosidase and alkaline phosphatase do not become extracellular when fused to the amino-terminal part of colicin N
A series of plasmids encoding hybrid proteins comprising various lengths of the NH2-terminal region of colicin N coupled to almost complete beta-galactosidase or alkaline phosphatase polypeptides was constructed by transposon mutagenesis of ColN plasmid de ...
1986