Concept
Subcloning
Publications associées (17)
Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells
Florian Maria Wurm, Bernard Schneider, Vivianne Padrun, Daniel Roman Blessing, Gabriel Vachey, Nicole Déglon
Adeno-associated virus (AAV) vectors are currently among the most commonly applied for in vivo gene therapy approaches. The evaluation of vectors during clinical development requires the production of considerable amounts of highly pure and potent vectors. ...
2019Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures
Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Stéphanie Tissot, Clara Joséphine Douet
We investigated the cultivation of bacterial, mammalian and insect cells in an orbitally-shaken 250-mL disposable tube that incorporates a helical track (HT) on its inside wall. The mass transfer coefficient of oxygen (kLa) was 200%–400% higher in the HT tu ...
2013Bioconjugates for bioseparation by affinity precipitation
Recent advances in the fields of biotechnology and medicine have intensified the necessity for pharmaceutical-grade biomacromolecules purification (e.g. enzymes, antibodies, plasmid DNA). One of the key factors for a successful downstream process is the po ...
EPFL2004