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Electron crystallography of membrane proteins determines the structure of membrane-reconstituted and two-dimensionally (2D) crystallized membrane proteins by low-dose imaging with the transmission electron microscope, and computer image processing. We have ...
The armadillo-related protein beta-catenin has multiple functions in cardiac tissue homeostasis: stabilization of beta-catenin has been implicated in adult cardiac hypertrophy, and downregulation initiates heart formation in embryogenesis. The protein is a ...
The molecular machinery responsible for the generation of transport carriers moving from the Golgi complex to the plasma membrane relies on a tight interplay between proteins and lipids. Among the lipid-binding proteins of this machinery, we previously ide ...
Stencil lithography is a surface patterning technique that relies on the local physical vapor deposition of material through miniaturized shadow mask membranes. It is extremely useful for the formation of patterns, mainly thin structured metal films, in si ...
The aim in this project was the optimization of LaFeO3-based perovskite materials for oxygen separation membranes (OSM). The work was carried out by the Laboratory for High Performance Ceramics, Empa in collaboration with the Laboratory for Industrial Ener ...
A simple but powerful numerical simulation for analyzing the electrochemical behavior of ion-selective membranes and liquid junctions is presented. The computer modeling makes use of a finite-difference procedure in the space and time domains, which can be ...
Scanning Electrochemical Microscopy (SECM) and copper staining are combined to achieve visualisation of proteins on surfaces. Proteins are adsorbed on a polyvinylidene fluoride (PVDF) membrane or on a polyethylene terephthalate (PET) surface and stained us ...
Special high temperature micro-hotplates were investigated for their potential use as a technology platform for miniaturized solid oxide fuel cells (SOFCs). To evaluate the compatibility of these hotplates with typical SOFC materials and processing techniq ...
In order to realize an implantable glucose sensing system designed within this project, the realization of a selective nanoporous membrane is essential. The final goal of this sub-project is establishing a fabrication process of a semi-permeable membrane c ...
The bacterial toxin aerolysin kills cells by forming heptameric channels, of unknown structure, in the plasma membrane. Using disulfide trapping and cysteine scanning mutagenesis coupled to thiol-specific labeling on lipid bilayers, we identify a loop that ...
