Concept

Polymorphisme de longueur des fragments amplifiés

Publications associées (4)

Lentiviral-mediated gene transfer into haematopoietic stem cells

OBJECTIVES: Lentiviral vectors can transduce nondividing cells. As most haematopoietic stem cells (HSCs) are nondividing in vivo, lentiviral vectors are promising viral vectors to transfer genes into HSCs. DESIGN AND SETTING: We have used HIV-1 based lenti ...

Wiley-Blackwell2001

Sampling the genomic pool of protein tyrosine kinase genes using the polymerase chain reaction with genomic DNA

Andrew Charles Oates

The polymerase chain reaction (PCR), with cDNA as template, has been widely used to identify members of protein families from many species. A major limitation of using cDNA in PCR is that detection of a family member is dependent on temporal and spatial pa ...

1998

Integration and stability of CHO amplicons containing plasmid sequences

Florian Maria Wurm

Fluorescence in situ hybridization (FISH, 15) and high density, non-selective long term perfusion culture were used to study aspects of genetic stability and productivity in recombinant CHO cells. We analysed the distribution and structure of recombinant a ...

1992

Use of fluorescence in situ hybridization to detect and monitor transfected and amplified sequences in recombinant CHO cells

Florian Maria Wurm

Non-radioactive fluorescence in situ hybridization was used to detect transfected and amplified sequences in recombinant CHO cells. The cell lines used in this study are based on DHFR-deficient CHO-DUKX cells. The recombinant CHO cells contain and express, ...

1989

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